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NK651

Plasmid

#109107

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Publication

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215 deltaCC

Use

Choanoflagellate expression

Mutation

Deletes C-terminal coiled-coil from PTSG_07215

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Publication

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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psPAX2_D64V_NC_eYFP

Plasmid

#257940

Purpose

For integrase-deficient, eYFP-labeled virus-like particle production in mammalian cells

Depositor

Jean-Laurent Casanova

Publication

Kim et al Bio Protoc. 2026 Jul 5;16(13):e5726. doi

Insert

gag pol

Use

Lentiviral; Packaging

Tags

eYFP Q69K

Expression

Mammalian

Available since

Aug. 17, 2026

Availability

Academic Institutions and Nonprofits only

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NBALFA (pDS1188)

Plasmid

#236357

Purpose

Anti-ALFA tag nanobody to be used as a part 3A in the MTK

Depositor

Hana El-Samad

Insert

NBALFA

Use

Synthetic Biology

Promoter

None

Available since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAJE90-Ma-tfmW-RSA2

Plasmid

#251520

Purpose

Machinery plasmid expressing Methanomethylophilus alvus aaRS/tRNA for TAG suppression and incorporation of trifluoromethyl-tryptophan (tfm-Trp) into sfGFP150TAG in E. coli

Depositor

Ryan Mehl

Publication

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Bacterial

Promoter

Gln S

Available since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAcBAc1-Ma-tfmW-RSA2

Plasmid

#251519

Purpose

Orthogonal Methanomethylophilus alvus aaRS/tRNA-mediated incorporation of tfm-Tryptophan into sfGFP150TAG in HEK293T cells

Depositor

Ryan Mehl

Publication

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3HA::tbTurboID::3HA

Plasmid

#247592

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3HA::FRB::3HA

Plasmid

#247587

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3HA::tbTurboID::3HA

Plasmid

#247591

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3HA::FRB::3HA

Plasmid

#247589

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-ICAM-5-Puro

Plasmid

#239949

Purpose

CRISPR KO of human ICAM-5

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

ICAM-5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-MFSD6-Puro

Plasmid

#239950

Purpose

CRISPR KO of human MFSD6

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPmTagBFP2FrFAST

Plasmid

#223839

Purpose

Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP2>FrFAST

Expression

Yeast

Promoter

TEF1

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Publication

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

LCB1_v1.3

Use

Synthetic Biology

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Publication

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

LCB3_v1.2

Use

Synthetic Biology

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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aCE89

Plasmid

#204494

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

Depositor

Ron Weiss

Publication

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

φC31 integrase
WBeta recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

PhIF and TREtight

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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aCE106

Plasmid

#204496

Purpose

Small molecule helper circuit

Depositor

Ron Weiss

Publication

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

rtTA3
PhIF-ABI
VPR-Pyl1

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Myc-mNG-3Myc

Plasmid

#190217

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Myc-mNG-3Myc

Plasmid

#190218

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Publication

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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