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pYPQ265E1

Plasmid

#164718

Purpose

Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a-cbe_v01 entry clone

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E3

Plasmid

#164720

Purpose

Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

eA3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7007

Plasmid

#136946

Purpose

Malachite green aptamer with stability hairpin, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ292

Plasmid

#102686

Purpose

S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - MPX

Plasmid

#89619

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Mitocondrial Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJK695

Plasmid

#71644

Purpose

Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)

Depositor

T. J. Kappock

Insert

phosphoribosylaminoimidazole carboxylase

Expression

Bacterial

Mutation

changes histidine-17 to tryptophan

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK696

Plasmid

#71645

Purpose

Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)

Depositor

T. J. Kappock

Insert

phosphoribosylaminoimidazole carboxylase

Expression

Bacterial

Mutation

changes methionine-15 to tryptophan

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHM148

Plasmid

#225532

Purpose

Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
Pcdh19 (Pcdh19 Mouse)
mCherry

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

WPRE terminator

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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aCE90

Plasmid

#204495

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

phiC31 recombinase
WBeta recombinase

Use

Synthetic Biology

Tags

PEST

Expression

Bacterial and Mammalian

Promoter

PhIF and TREtight

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Magenta

Plasmid

#183140

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Magenta

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Yellow

Plasmid

#183141

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Yellow

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Yellow

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Orange

Plasmid

#183143

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Orange

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Pink

Plasmid

#183142

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Pink

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0808

Plasmid

#177082

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

tryptophan decarboxylase (CrTDC) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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DT040A

Plasmid

#159401

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

hybrid J23117-J23106 promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJK648

Plasmid

#71611

Purpose

Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)

Depositor

T. J. Kappock

Insert

phosphoribosylaminoimidazole carboxylase

Expression

Bacterial

Mutation

changes phenylalanine-79 to tryptophan

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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