We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

   Plasmid

   #50888

   Purpose

   Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. NC12 pCDNA3.1 VENUS WTX delta ETGE

   Plasmid

   #36967

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX (delta ETGE motif) (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   lacks amino acids 288 –291

   Promoter

   CMV

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP

   Plasmid

   #239769

   Purpose

   Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K1435TAG, Y371C

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)

   Plasmid

   #238430

   Purpose

   Expresses GRP78 AAA mutant without the UTRs fused to FLAG tag

   Depositor

   Amy Shiu Lee

   Article

   Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The UTRs of the gene have been deleted. The PPP t…

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)

   Plasmid

   #238429

   Purpose

   Expresses GRP78 Full Length without UTRs fused to the FLAG Tag

   Depositor

   Amy Shiu Lee

   Article

   Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The UTRs of the gene have been deleted.

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

   Plasmid

   #216491

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1
   

   Use

   Tags

   NES, T2A-EGFP-CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

   Plasmid

   #233065

   Purpose

   Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Leucine 928 to Alanine; Methionine 929 to Alanine

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

   Plasmid

   #227615

   Purpose

   Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Use

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   G249D

   Promoter

   CMV, T7

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HA-GTSE1-S91, 262 ,331, 724A

   Plasmid

   #224738

   Purpose

   For expression of HA-tagged GTSE1 protein with S91, 262 ,331, 724A mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Serine 91, 262 ,331, 724 to Alanine

   Promoter

   CMV

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid

    #228530

    Purpose

    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Photinus pyralis, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11)

    Plasmid

    #227616

    Purpose

    Overexpression of SLC25A46 with R257Q mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257Q

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

    Plasmid

    #227617

    Purpose

    Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R340C

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

    Plasmid

    #227618

    Purpose

    Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    I349D

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

    Plasmid

    #187725

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

    Plasmid

    #187726

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

    Plasmid

    #215900

    Purpose

    Expression of TNRC6A-CIM1 domain with S1332A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1332A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

    Plasmid

    #215901

    Purpose

    Expression of TNRC6A-CIM1 domain with S1346A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1346A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A

    Plasmid

    #215903

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A

    Plasmid

    #215904

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1616A, S1691A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)

    Plasmid

    #224282

    Purpose

    Cell transfection and expression of Rac1 (G12C, K96H, P29S)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C, K96H, P29S

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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