We narrowed to 259 results for: T7 rna polymerase

1. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS4

   Plasmid

   #55751

   Purpose

   Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 1-59 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. rFIXX-bp chain A (IRESneo)

   Plasmid

   #82450

   Purpose

   Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.

   Depositor

   Robert Peters

   Insert

   rFIXX-bp chain A
   

   Tags

   Polyhistidine

   Expression

   Bacterial and Mammalian

   Promoter

   T7 RNA polymerase promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. rFIXX-bp chain B (IRESpuro)

   Plasmid

   #82451

   Purpose

   Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.

   Depositor

   Robert Peters

   Insert

   rFIXX-bp chain B
   

   Tags

   Biotin

   Expression

   Bacterial and Mammalian

   Promoter

   T7 RNA polymerase promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2,0_3M5M_luciferase

   Plasmid

   #135475

   Purpose

   Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, luciferase reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJP_Azul01

   Plasmid

   #219670

   Purpose

   Blue light inducible system

   Depositor

   Yolanda Schaerli

   Article

   Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

   Inserts

   similar to T7 RNA polymerase (core)
   similar to YF1-fixJ
   

   Use

   Synthetic Biology

   Promoter

   LacIq35 and pJ23105

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB8589

    Plasmid

    #175221

    Purpose

    Xenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YMR253C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8707

    Plasmid

    #175222

    Purpose

    Xenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YNL095C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB8728

    Plasmid

    #175223

    Purpose

    Xenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL060C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB8709

    Plasmid

    #175224

    Purpose

    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL092W (CEN.PK strain)
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB8711

    Plasmid

    #175225

    Purpose

    Xenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR092W
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB8713

    Plasmid

    #175226

    Purpose

    Xenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR271C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8590

    Plasmid

    #175227

    Purpose

    Xenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR307C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB8738

    Plasmid

    #175228

    Purpose

    Xenopus oocyte expression vector containing YPL189W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL189W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YPL189W
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB8591

    Plasmid

    #175229

    Purpose

    Xenopus oocyte expression vector containing YPL244C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL244C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YPL244C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB8592

    Plasmid

    #175230

    Purpose

    Xenopus oocyte expression vector containing YPL264C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL264C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YPL264C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB8715

    Plasmid

    #175231

    Purpose

    Xenopus oocyte expression vector containing YPR201W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPR201W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YPR201W
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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