165,191 results

1. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAT17

   Plasmid

   #204298

   Purpose

   For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EYFP
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

   Plasmid

   #83842

   Purpose

   Fluorescent probe for S/G2 cell cycle transition and G2/M transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220727

   Purpose

   AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Bosiljka Tasic, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

   Plasmid

   #140001

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA-Sumo1 WT

   Plasmid

   #48966

   Purpose

   Expresses HA-Sumo1 WT in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO1 (SUMO1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-p65

    Plasmid

    #111190

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    RELA (RELA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-YE1-BE4max

    Plasmid

    #198546

    Purpose

    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    lac promoter / tac promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiPGK Puro DEST JNKKTRClover

    Plasmid

    #59151

    Purpose

    Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. TDP-43_CTD

    Plasmid

    #98669

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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