166,708 results

1. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Expression

   Bacterial

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC70202

   Plasmid

   #209463

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Promoter

   TaU6

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLRP3-mNeonGreen

   Plasmid

   #200942

   Purpose

   NLRP3 fused with mNeonGreen to indicate its localization change

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTransposon.CAG.CasRX-P2A-Blast

   Plasmid

   #212965

   Purpose

   Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   CasRx
   

   Use

   Transposon

   Tags

   P2A-Blasticidin resistance

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAH-POLY-eRF1(E55D)

   Plasmid

   #157925

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

   Insert

   POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLS-mP

   Plasmid

   #81225

   Purpose

   Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   Derived from pGL4.23

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-PRKD1

   Plasmid

   #23500

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PRKD1 (PRKD1 Human)
   

   Use

   Gateway donor vector

   Mutation

   1MM

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qMaLioffG

    Plasmid

    #212825

    Purpose

    green fluorescent FLIM indicator for endogenous ATP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al bioRxiv 2023.08.29.555275

    Insert

    qMaLioffG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-AXL

    Plasmid

    #116714

    Purpose

    Lentiviral expression of AXL

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLXSN16E6E7

    Plasmid

    #52394

    Purpose

    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV16 E6E7
    

    Use

    Retroviral

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Dendra2

    Plasmid

    #103967

    Purpose

    Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    Dendra2
    

    Expression

    Mammalian

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBiT3.1-N_DCAF11:1-546

    Plasmid

    #211197

    Purpose

    Mammalian expression of full-length human DCAF11. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF11:M1-Q546 (DCAF11 Human)
    

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    R207H natural variant (VAR_020121)

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-P-TAG (pGS594)

    Plasmid

    #160039

    Purpose

    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    CP450TMD-APEX2-3xHA
    

    Use

    Lentiviral

    Promoter

    EF1

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

    Plasmid

    #60661

    Purpose

    Flp-dependent expression of Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX- Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Use

    AAV

    Promoter

    EF1α

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Promoter

    None

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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