166,778 results

1. pIB2

   Plasmid

   #25451

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hGFAP-mCherry

   Plasmid

   #178581

   Purpose

   Expression of mCherry under the human GFAP promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTU-DO-HPH-C4

   Plasmid

   #160343

   Purpose

   High-copy expression vector for K. marxianus, hygromycin selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-RNaseHI-NES-EGFP

   Plasmid

   #196701

   Purpose

   Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   bacterial RNaseHI
   

   Use

   Lentiviral

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pb-TRE3G-SpCas9-IRES-blast

   Plasmid

   #195506

   Purpose

   Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNL [NlucP/STAT6-RE/Hygro] Vector

   Plasmid

   #236925

   Purpose

   Express STAT6 response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   STAT6 (STAT6 Human)
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LV_EF1a_CDK4-P2A-Hygro_Barcode

   Plasmid

   #170223

   Purpose

   Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR15.2-2H12.D11opt

   Plasmid

   #221185

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2.1
   

   Expression

   Bacterial

   Promoter

   Constitutive

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE2-SpG

    Plasmid

    #159978

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE_hygro_mRFP1_NRF2

    Plasmid

    #136579

    Purpose

    mammalian expression of mRFP1-tagged human NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-nSpCas9-APOBEC1

    Plasmid

    #209039

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-hSyn-nucGFP

    Plasmid

    #140190

    Purpose

    Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    GFP-NLS
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 35S-sfGFP-nosT

    Plasmid

    #80129

    Purpose

    Transient expression vector for sfGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CARPID dCasRx-BASU

    Plasmid

    #153303

    Purpose

    express CARPID dCasRx-BASU fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    dCasRx-BASU
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Expression

    Plant

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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