Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEGFP-LC3 Citations (61)

Plasmid Article: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T EMBO J. 2000 Nov 1. 19(21):5720-8.
PubMed Journal

Articles Citing pEGFP-LC3

Articles
Depletion of L-arginine induces autophagy as a cytoprotective response to endoplasmic reticulum stress in human T lymphocytes. Garcia-Navas R, Munder M, Mollinedo F. Autophagy. 2012 Nov;8(11):1557-76. doi: 10.4161/auto.21315. Epub 2012 Aug 9. PubMed
Caffeine stimulates hepatic lipid metabolism by the autophagy-lysosomal pathway in mice. Sinha RA, Farah BL, Singh BK, Siddique MM, Li Y, Wu Y, Ilkayeva OR, Gooding J, Ching J, Zhou J, Martinez L, Xie S, Bay BH, Summers SA, Newgard CB, Yen PM. Hepatology. 2014 Apr;59(4):1366-80. doi: 10.1002/hep.26667. Epub 2014 Feb 18. PubMed
Francisella tularensis harvests nutrients derived via ATG5-independent autophagy to support intracellular growth. Steele S, Brunton J, Ziehr B, Taft-Benz S, Moorman N, Kawula T. PLoS Pathog. 2013 Aug;9(8):e1003562. doi: 10.1371/journal.ppat.1003562. Epub 2013 Aug 15. PubMed
Impairment of autophagic flux promotes glucose reperfusion-induced neuro2A cell death after glucose deprivation. Jang BG, Choi BY, Kim JH, Kim MJ, Sohn M, Suh SW. PLoS One. 2013 Oct 4;8(10):e76466. doi: 10.1371/journal.pone.0076466. eCollection 2013. PubMed
BNIP3 plays crucial roles in the differentiation and maintenance of epidermal keratinocytes. Moriyama M, Moriyama H, Uda J, Matsuyama A, Osawa M, Hayakawa T. J Invest Dermatol. 2014 Jun;134(6):1627-35. doi: 10.1038/jid.2014.11. Epub 2014 Jan 8. PubMed
Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses. Santiago FW, Covaleda LM, Sanchez-Aparicio MT, Silvas JA, Diaz-Vizarreta AC, Patel JR, Popov V, Yu XJ, Garcia-Sastre A, Aguilar PV. J Virol. 2014 Apr;88(8):4572-85. doi: 10.1128/JVI.03021-13. Epub 2014 Jan 29. PubMed
Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance. Zhou J, Farah BL, Sinha RA, Wu Y, Singh BK, Bay BH, Yang CS, Yen PM. PLoS One. 2014 Jan 29;9(1):e87161. doi: 10.1371/journal.pone.0087161. eCollection 2014. PubMed
The combination of rapamycin and MAPK inhibitors enhances the growth inhibitory effect on Nara-H cells. Nakamura O, Hitora T, Yamagami Y, Mori M, Nishimura H, Horie R, Yamaguchi K, Yamamoto T. Int J Mol Med. 2014 Jun;33(6):1491-7. doi: 10.3892/ijmm.2014.1715. Epub 2014 Mar 27. PubMed
Activation of ERK by sodium tungstate induces protein synthesis and prevents protein degradation in rat L6 myotubes. Salto R, Vilchez JD, Cabrera E, Guinovart JJ, Giron MD. FEBS Lett. 2014 Jun 27;588(14):2246-54. doi: 10.1016/j.febslet.2014.05.004. Epub 2014 May 17. PubMed
beta-Adrenergic agonist and antagonist regulation of autophagy in HepG2 cells, primary mouse hepatocytes, and mouse liver. Farah BL, Sinha RA, Wu Y, Singh BK, Zhou J, Bay BH, Yen PM. PLoS One. 2014 Jun 20;9(6):e98155. doi: 10.1371/journal.pone.0098155. eCollection 2014. PubMed
Formation of alpha-synuclein Lewy neurite-like aggregates in axons impedes the transport of distinct endosomes. Volpicelli-Daley LA, Gamble KL, Schultheiss CE, Riddle DM, West AB, Lee VM. Mol Biol Cell. 2014 Dec 15;25(25):4010-23. doi: 10.1091/mbc.E14-02-0741. Epub 2014 Oct 8. PubMed
ROS-dependent prostate apoptosis response-4 (Par-4) up-regulation and ceramide generation are the prime signaling events associated with curcumin-induced autophagic cell death in human malignant glioma. Thayyullathil F, Rahman A, Pallichankandy S, Patel M, Galadari S. FEBS Open Bio. 2014 Aug 30;4:763-76. doi: 10.1016/j.fob.2014.08.005. eCollection 2014. PubMed
Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium. Toops KA, Tan LX, Jiang Z, Radu RA, Lakkaraju A. Mol Biol Cell. 2015 Jan 1;26(1):1-14. doi: 10.1091/mbc.E14-05-1028. Epub 2014 Nov 5. PubMed
Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy. Van Laar VS, Roy N, Liu A, Rajprohat S, Arnold B, Dukes AA, Holbein CD, Berman SB. Neurobiol Dis. 2015 Feb;74:180-93. doi: 10.1016/j.nbd.2014.11.015. Epub 2014 Dec 3. PubMed
Obatoclax induces Beclin 1- and ATG5-dependent apoptosis and autophagy in adenoid cystic carcinoma cells. Liang LZ, Ma B, Liang YJ, Liu HC, Zhang TH, Zheng GS, Su YX, Liao GQ. Oral Dis. 2015 May;21(4):470-7. doi: 10.1111/odi.12305. Epub 2015 Jan 19. PubMed
beta-Hydroxy-beta-methylbutyrate (HMB) normalizes dexamethasone-induced autophagy-lysosomal pathway in skeletal muscle. Giron MD, Vilchez JD, Shreeram S, Salto R, Manzano M, Cabrera E, Campos N, Edens NK, Rueda R, Lopez-Pedrosa JM. PLoS One. 2015 Feb 6;10(2):e0117520. doi: 10.1371/journal.pone.0117520. eCollection 2015. PubMed
Control of autophagosome axonal retrograde flux by presynaptic activity unveiled using botulinum neurotoxin type a. Wang T, Martin S, Papadopulos A, Harper CB, Mavlyutov TA, Niranjan D, Glass NR, Cooper-White JJ, Sibarita JB, Choquet D, Davletov B, Meunier FA. J Neurosci. 2015 Apr 15;35(15):6179-94. doi: 10.1523/JNEUROSCI.3757-14.2015. PubMed
AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Herms A, Bosch M, Reddy BJ, Schieber NL, Fajardo A, Ruperez C, Fernandez-Vidal A, Ferguson C, Rentero C, Tebar F, Enrich C, Parton RG, Gross SP, Pol A. Nat Commun. 2015 May 27;6:7176. doi: 10.1038/ncomms8176. PubMed
Thyroid hormone induction of mitochondrial activity is coupled to mitophagy via ROS-AMPK-ULK1 signaling. Sinha RA, Singh BK, Zhou J, Wu Y, Farah BL, Ohba K, Lesmana R, Gooding J, Bay BH, Yen PM. Autophagy. 2015;11(8):1341-57. doi: 10.1080/15548627.2015.1061849. PubMed
Cordycepin induces apoptosis and autophagy in human neuroblastoma SK-N-SH and BE(2)-M17 cells. Li Y, Li R, Zhu S, Zhou R, Wang L, DU J, Wang Y, Zhou B, Mai L. Oncol Lett. 2015 Jun;9(6):2541-2547. Epub 2015 Mar 23. PubMed
Withaferin A Induces Cell Death Selectively in Androgen-Independent Prostate Cancer Cells but Not in Normal Fibroblast Cells. Nishikawa Y, Okuzaki D, Fukushima K, Mukai S, Ohno S, Ozaki Y, Yabuta N, Nojima H. PLoS One. 2015 Jul 31;10(7):e0134137. doi: 10.1371/journal.pone.0134137. eCollection 2015. PubMed
Induction of autophagy improves hepatic lipid metabolism in glucose-6-phosphatase deficiency. Farah BL, Landau DJ, Sinha RA, Brooks ED, Wu Y, Fung SY, Tanaka T, Hirayama M, Bay BH, Koeberl DD, Yen PM. J Hepatol. 2016 Feb;64(2):370-9. doi: 10.1016/j.jhep.2015.10.008. Epub 2015 Oct 20. PubMed
Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle. Lesmana R, Sinha RA, Singh BK, Zhou J, Ohba K, Wu Y, Yau WW, Bay BH, Yen PM. Endocrinology. 2016 Jan;157(1):23-38. doi: 10.1210/en.2015-1632. Epub 2015 Nov 12. PubMed
The Retinoblastoma Tumor Suppressor Protein (pRb)/E2 Promoter Binding Factor 1 (E2F1) Pathway as a Novel Mediator of TGFbeta-induced Autophagy. Korah J, Canaff L, Lebrun JJ. J Biol Chem. 2016 Jan 29;291(5):2043-54. doi: 10.1074/jbc.M115.678557. Epub 2015 Nov 23. PubMed
Cellular localization and effects of ectopically expressed hepatitis A virus proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC. Seggewiss N, Kruse HV, Weilandt R, Domsgen E, Dotzauer A, Paulmann D. Arch Virol. 2015 Dec 28. PubMed
Group IVA phospholipase A(2) deficiency prevents CCl4-induced hepatic cell death through the enhancement of autophagy. Ishihara K, Kanai S, Tanaka K, Kawashita E, Akiba S. Biochem Biophys Res Commun. 2016 Feb 26;471(1):15-20. doi: 10.1016/j.bbrc.2016.01.186. Epub 2016 Feb 3. PubMed
Autophagy induction by SIRT6 is involved in oxidative stress-induced neuronal damage. Shao J, Yang X, Liu T, Zhang T, Xie QR, Xia W. Protein Cell. 2016 Apr;7(4):281-90. doi: 10.1007/s13238-016-0257-6. Epub 2016 Mar 16. PubMed
Mediation of Autophagic Cell Death by Type 3 Ryanodine Receptor (RyR3) in Adult Hippocampal Neural Stem Cells. Chung KM, Jeong EJ, Park H, An HK, Yu SW. Front Cell Neurosci. 2016 May 6;10:116. doi: 10.3389/fncel.2016.00116. eCollection 2016. PubMed
SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages. Kim MJ, Bae SH, Ryu JC, Kwon Y, Oh JH, Kwon J, Moon JS, Kim K, Miyawaki A, Lee MG, Shin J, Kim YS, Kim CH, Ryter SW, Choi AM, Rhee SG, Ryu JH, Yoon JH. Autophagy. 2016 Aug 2;12(8):1272-91. doi: 10.1080/15548627.2016.1183081. Epub 2016 Jun 23. PubMed
LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes. Hubert V, Peschel A, Langer B, Groger M, Rees A, Kain R. Biol Open. 2016 Oct 15;5(10):1516-1529. doi: 10.1242/bio.018648. PubMed
Silymarin attenuates cigarette smoke extract-induced inflammation via simultaneous inhibition of autophagy and ERK/p38 MAPK pathway in human bronchial epithelial cells. Li D, Hu J, Wang T, Zhang X, Liu L, Wang H, Wu Y, Xu D, Wen F. Sci Rep. 2016 Nov 22;6:37751. doi: 10.1038/srep37751. PubMed
BNIP3 upregulation via stimulation of ERK and JNK activity is required for the protection of keratinocytes from UVB-induced apoptosis. Moriyama M, Moriyama H, Uda J, Kubo H, Nakajima Y, Goto A, Morita T, Hayakawa T. Cell Death Dis. 2017 Feb 2;8(2):e2576. doi: 10.1038/cddis.2017.4. PubMed
Autophagy Activation by Rapamycin Before Hypoxia-Reoxygenation Reduces Endoplasmic Reticulum Stress in Alveolar Epithelial Cells. Fan T, Chen L, Huang Z, Wang W, Zhang B, Xu Y, Mao Z, Hu H, Geng Q. Cell Physiol Biochem. 2017;41(1):79-90. doi: 10.1159/000455953. Epub 2017 Jan 17. PubMed
Bassoon Controls Presynaptic Autophagy through Atg5. Okerlund ND, Schneider K, Leal-Ortiz S, Montenegro-Venegas C, Kim SA, Garner LC, Waites CL, Gundelfinger ED, Reimer RJ, Garner CC. Neuron. 2017 Feb 22;93(4):897-913.e7. doi: 10.1016/j.neuron.2017.01.026. PubMed
Carbon monoxide protects against hepatic steatosis in mice by inducing sestrin-2 via the PERK-eIF2alpha-ATF4 pathway. Kim HJ, Joe Y, Kim SK, Park SU, Park J, Chen Y, Kim J, Ryu J, Cho GJ, Surh YJ, Ryter SW, Kim UH, Chung HT. Free Radic Biol Med. 2017 Sep;110:81-91. doi: 10.1016/j.freeradbiomed.2017.05.026. Epub 2017 May 31. PubMed
Phosphorylation of p62 by AMP-activated protein kinase mediates autophagic cell death in adult hippocampal neural stem cells. Ha S, Jeong SH, Yi K, Chung KM, Hong CJ, Kim SW, Kim EK, Yu SW. J Biol Chem. 2017 Jun 27. pii: jbc.M117.780874. doi: 10.1074/jbc.M117.780874. PubMed
Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin. Xiao B, Goh JY, Xiao L, Xian H, Lim KL, Liou YC. J Biol Chem. 2017 Oct 6;292(40):16697-16708. doi: 10.1074/jbc.M117.787739. Epub 2017 Aug 28. PubMed
Superoxide drives progression of Parkin/PINK1-dependent mitophagy following translocation of Parkin to mitochondria. Xiao B, Deng X, Lim GGY, Xie S, Zhou ZD, Lim KL, Tan EK. Cell Death Dis. 2017 Oct 12;8(10):e3097. doi: 10.1038/cddis.2017.463. PubMed
Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance. Lin SY, Hsieh SY, Fan YT, Wei WC, Hsiao PW, Tsai DH, Wu TS, Yang NS. Autophagy. 2018;14(5):778-795. doi: 10.1080/15548627.2017.1386359. Epub 2017 Dec 31. PubMed
Autophagy induction by Mycobacterium indicus pranii promotes Mycobacterium tuberculosis clearance from RAW 264.7 macrophages. Singh B, Saqib M, Gupta A, Kumar P, Bhaskar S. PLoS One. 2017 Dec 13;12(12):e0189606. doi: 10.1371/journal.pone.0189606. eCollection 2017. PubMed
Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways. Mizunoe Y, Kobayashi M, Sudo Y, Watanabe S, Yasukawa H, Natori D, Hoshino A, Negishi A, Okita N, Komatsu M, Higami Y. Redox Biol. 2018 May;15:115-124. doi: 10.1016/j.redox.2017.09.007. Epub 2017 Sep 20. PubMed
Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells. Kang YT, Hsu WC, Wu CH, Hsin IL, Wu PR, Yeh KT, Ko JL. Oncotarget. 2017 Nov 6;8(62):105536-105552. doi: 10.18632/oncotarget.22317. eCollection 2017 Dec 1. PubMed
Drug Repurposing Screening Identifies Tioconazole as an ATG4 Inhibitor that Suppresses Autophagy and Sensitizes Cancer Cells to Chemotherapy. Liu PF, Tsai KL, Hsu CJ, Tsai WL, Cheng JS, Chang HW, Shiau CW, Goan YG, Tseng HH, Wu CH, Reed JC, Yang LW, Shu CW. Theranostics. 2018 Jan 1;8(3):830-845. doi: 10.7150/thno.22012. eCollection 2018. PubMed
Antimycobacterial effect of IFNG (interferon gamma)-induced autophagy depends on HMOX1 (heme oxygenase 1)-mediated increase in intracellular calcium levels and modulation of PPP3/calcineurin-TFEB (transcription factor EB) axis. Singh N, Kansal P, Ahmad Z, Baid N, Kushwaha H, Khatri N, Kumar A. Autophagy. 2018;14(6):972-991. doi: 10.1080/15548627.2018.1436936. Epub 2018 May 10. PubMed
Taurine is an amino acid with the ability to activate autophagy in adipocytes. Kaneko H, Kobayashi M, Mizunoe Y, Yoshida M, Yasukawa H, Hoshino S, Itagawa R, Furuichi T, Okita N, Sudo Y, Imae M, Higami Y. Amino Acids. 2018 May;50(5):527-535. doi: 10.1007/s00726-018-2550-6. Epub 2018 Mar 9. PubMed
Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Duan X, Kong Z, Mai X, Lan Y, Liu Y, Yang Z, Zhao Z, Deng T, Zeng T, Cai C, Li S, Zhong W, Wu W, Zeng G. Redox Biol. 2018 Jun;16:414-425. doi: 10.1016/j.redox.2018.03.019. Epub 2018 Apr 2. PubMed
Saturated fatty acids promote chondrocyte matrix remodeling through reprogramming of autophagy pathways. Sekar S, Wu X, Friis T, Crawford R, Prasadam I, Xiao Y. Nutrition. 2018 Oct;54:144-152. doi: 10.1016/j.nut.2018.02.018. Epub 2018 Mar 20. PubMed
NorUDCA promotes degradation of alpha1-antitrypsin mutant Z protein by inducing autophagy through AMPK/ULK1 pathway. Tang Y, Blomenkamp KS, Fickert P, Trauner M, Teckman JH. PLoS One. 2018 Aug 1;13(8):e0200897. doi: 10.1371/journal.pone.0200897. eCollection 2018. PubMed
Deep Analysis of Mitochondria and Cell Health Using Machine Learning. Zahedi A, On V, Phandthong R, Chaili A, Remark G, Bhanu B, Talbot P. Sci Rep. 2018 Nov 5;8(1):16354. doi: 10.1038/s41598-018-34455-y. PubMed
TLR4 (toll-like receptor 4) activation suppresses autophagy through inhibition of FOXO3 and impairs phagocytic capacity of microglia. Lee JW, Nam H, Kim LE, Jeon Y, Min H, Ha S, Lee Y, Kim SY, Lee SJ, Kim EK, Yu SW. Autophagy. 2019 May;15(5):753-770. doi: 10.1080/15548627.2018.1556946. Epub 2018 Dec 13. PubMed
PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide. Ming K, Yuan W, Chen Y, Du H, He M, Hu Y, Wang D, Wu Y, Liu J. Carbohydr Polym. 2019 Mar 15;208:22-31. doi: 10.1016/j.carbpol.2018.12.035. Epub 2018 Dec 13. PubMed
Assays for Neuronal Defects Caused by Early Formation of alpha-Synuclein Inclusions in Primary Cultured Neurons. Volpicelli-Daley LA. Methods Mol Biol. 2019;1948:1-14. doi: 10.1007/978-1-4939-9124-2_1. PubMed
Keratinocyte differentiation promotes ER stress-dependent lysosome biogenesis. Mahanty S, Dakappa SS, Shariff R, Patel S, Swamy MM, Majumdar A, Setty SRG. Cell Death Dis. 2019 Mar 19;10(4):269. doi: 10.1038/s41419-019-1478-4. PubMed
Glycan sulfation patterns define autophagy flux at axon tip via PTPRsigma-cortactin axis. Sakamoto K, Ozaki T, Ko YC, Tsai CF, Gong Y, Morozumi M, Ishikawa Y, Uchimura K, Nadanaka S, Kitagawa H, Zulueta MML, Bandaru A, Tamura JI, Hung SC, Kadomatsu K. Nat Chem Biol. 2019 Jul;15(7):699-709. doi: 10.1038/s41589-019-0274-x. Epub 2019 May 6. PubMed
SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity. Zhang T, Liu J, Shen S, Tong Q, Ma X, Lin L. Cell Death Differ. 2019 Jun 3. pii: 10.1038/s41418-019-0356-z. doi: 10.1038/s41418-019-0356-z. PubMed
BAP31 regulates mitochondrial function via interaction with Tom40 within ER-mitochondria contact sites. Namba T. Sci Adv. 2019 Jun 12;5(6):eaaw1386. doi: 10.1126/sciadv.aaw1386. eCollection 2019 Jun. PubMed
Activation of the autophagy pathway by Torovirus infection is irrelevant for virus replication. Avila-Perez G, Diaz-Beneitez E, Cubas-Gaona LL, Nieves-Molina G, Rodriguez JR, Rodriguez JF, Rodriguez D. PLoS One. 2019 Jul 15;14(7):e0219428. doi: 10.1371/journal.pone.0219428. eCollection 2019. PubMed
Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Denu RA, Kaur G, Sass MM, Lakkaraju A, Burkard ME. Mol Cancer Res. 2020 Jan;18(1):33-45. doi: 10.1158/1541-7786.MCR-19-0509. Epub 2019 Oct 11. PubMed
BAP31 Inhibits Cell Adaptation to ER Stress Conditions, Negatively Regulating Autophagy Induction by Interaction with STX17. Machihara K, Namba T. Cells. 2019 Oct 30;8(11). pii: cells8111350. doi: 10.3390/cells8111350. PubMed
Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3. Thayyullathil F, Cheratta AR, Pallichankandy S, Subburayan K, Tariq S, Rangnekar VM, Galadari S. Biochim Biophys Acta Mol Cell Res. 2020 Jul;1867(7):118692. doi: 10.1016/j.bbamcr.2020.118692. Epub 2020 Mar 2. PubMed
The inhibitory effect of phosphorylated Codonopsis pilosula polysaccharide on autophagosomes formation contributes to the inhibition of duck hepatitis A virus replication. Ming K, He M, Su L, Du H, Wang D, Wu Y, Liu J. Poult Sci. 2020 Apr;99(4):2146-2156. doi: 10.1016/j.psj.2019.11.060. Epub 2020 Feb 8. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.