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pET-28b-Cas9-His Citations (12)

Plasmid Article: Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014.
PubMed Journal

Articles Citing pET-28b-Cas9-His

Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing. Mout R, Ray M, Yesilbag Tonga G, Lee YW, Tay T, Sasaki K, Rotello VM. ACS Nano. 2017 Mar 28;11(3):2452-2458. doi: 10.1021/acsnano.6b07600. Epub 2017 Jan 31. PubMed
A right-handed signalling pathway drives heart looping in vertebrates. Ocana OH, Coskun H, Minguillon C, Murawala P, Tanaka EM, Galceran J, Munoz-Chapuli R, Nieto MA. Nature. 2017 Sep 6;549(7670):86-90. doi: 10.1038/nature23454. PubMed
Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques. Ringer KP, Roth MG, Garey MS, Piorczynski TB, Suli A, Hansen JM, Alder JK. Cell Biol Int. 2018 Jul;42(7):849-858. doi: 10.1002/cbin.10952. Epub 2018 Mar 14. PubMed
A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins. Letelier J, de la Calle-Mustienes E, Pieretti J, Naranjo S, Maeso I, Nakamura T, Pascual-Anaya J, Shubin NH, Schneider I, Martinez-Morales JR, Gomez-Skarmeta JL. Nat Genet. 2018 Apr;50(4):504-509. doi: 10.1038/s41588-018-0080-5. Epub 2018 Mar 19. PubMed
Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure. Swinburne IA, Mosaliganti KR, Upadhyayula S, Liu TL, Hildebrand DGC, Tsai TY, Chen A, Al-Obeidi E, Fass AK, Malhotra S, Engert F, Lichtman JW, Kirchhausen T, Betzig E, Megason SG. Elife. 2018 Jun 19;7. pii: 37131. doi: 10.7554/eLife.37131. PubMed
DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes. Murovec J, Gucek K, Bohanec B, Avbelj M, Jerala R. Front Plant Sci. 2018 Nov 5;9:1594. doi: 10.3389/fpls.2018.01594. eCollection 2018. PubMed
Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, Aryee MJ, Joung JK. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. PubMed

Associated Plasmids

Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Warner BK, Alder JK, Suli A. Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16. PubMed
On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains. Carmignotto GP, Azzoni AR. J Biotechnol. 2019 Dec 20;306:62-70. doi: 10.1016/j.jbiotec.2019.09.012. Epub 2019 Sep 20. PubMed
Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Reuven N, Adler J, Broennimann K, Myers N, Shaul Y. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed

Associated Plasmids

An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms. Yu Y, Guo Y, Tian Q, Lan Y, Yeh H, Zhang M, Tasan I, Jain S, Zhao H. Nat Chem Biol. 2020 Apr;16(4):387-390. doi: 10.1038/s41589-019-0432-1. Epub 2019 Dec 23. PubMed
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. Saha C, Mohanraju P, Stubbs A, Dugar G, Hoogstrate Y, Kremers GJ, van Cappellen WA, Horst-Kreft D, Laffeber C, Lebbink JHG, Bruens S, Gaskin D, Beerens D, Klunder M, Joosten R, Demmers JAA, van Gent D, Mouton JW, van der Spek PJ, van der Oost J, van Baarlen P, Louwen R. Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi: 10.1126/sciadv.aaz4849. eCollection 2020 Jun. PubMed

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