Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF
PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. PubMed
Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF
PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47322 | pCS2-Cas9 | For in vivo expression of Cas9 or generation of Cas9 mRNA |
| 47327 | pET-28b-Cas9-His | For in vitro expression and purification of Cas9 protein |