162,717 results

1. pHCMV-SS-antiALFAtagNb-TM

   Plasmid

   #198307

   Purpose

   Displays anti-ALFA nanobody from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiALFAtagNb-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. c-myc promoter (Del 3)

   Plasmid

   #16603

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 3) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-3 (AccI->PvuII). The AccI site is at -741 …

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pUC-MLXγ

   Plasmid

   #211755

   Purpose

   Cloning vector for the expression of mouse MLXγ

   Depositor

   Russell Goodman

   Article

   Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

   Insert

   MLX (Mlx Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-rInsp-hIns-GLuc

   Plasmid

   #89927

   Purpose

   Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   410bp rat insulin II promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Opto-mFGFR1_204

   Plasmid

   #58745

   Purpose

   Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

   Depositor

   Harald Janovjak

   Article

   Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

   Insert

   Opto-mFGFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRIM28 gRNA (BRDN0001162487)

   Plasmid

   #76139

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

   Plasmid

   #72638

   Purpose

   Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain

   Depositor

   Joshua Bonkowsky

   Insert

   UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_nLightG

   Plasmid

   #217656

   Purpose

   Expresses norepinephrine indicator nLightG under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JD638

   Plasmid

   #160395

   Purpose

   Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   Vitis miR396-resistant GRF4-GIF1 chimera
   

   Use

   Entry clon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459-SpCas9-NG

    Plasmid

    #171370

    Purpose

    SpCas9-NG-mediated genome editing.

    Depositor

    Masahito Ikawa

    Article

    Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA; CBh for Cas9-2A-puroR

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-UAS-anti-CD3/anti-CD19 BiTE

    Plasmid

    #230005

    Purpose

    Gal4-dependent expression of secreted BiTE

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    UAS-anti-CD3/anti-CD19 BiTE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-TRIM28deltaRING

    Plasmid

    #124952

    Purpose

    Mammalian expression of Human TRIM28 with deleted RING domain

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RING domain

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

    Plasmid

    #155125

    Purpose

    Tol2 vector containing Cre-inducible QFGal4-switch

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4-switch
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Ubi

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMKLR1-Tango

    Plasmid

    #66253

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CMKLR1 (CMKLR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-ATXN1-2Q

    Plasmid

    #33239

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0623

    Plasmid

    #142666

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DDIT3 (DDIT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRIM28 gRNA (BRDN0001146162)

    Plasmid

    #76142

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNL(CMV)TK2ALuc2/CMV/WPREdU3

    Plasmid

    #163965

    Purpose

    Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    HSV-1 TK and FLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCMV-SS-antiEEscFv-TM

    Plasmid

    #198304

    Purpose

    Displays anti-EE scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiEEscFv-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge