166,778 results

1. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

   Plasmid

   #176531

   Purpose

   PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

   Depositor

   Jared Toettcher

   Article

   Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

   Inserts

   KGV transcription factor
   TagBFP
   

   Use

   Piggybac transposase integration vector

   Tags

   ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

   Expression

   Mammalian

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR-v1-sgGLS_2

   Plasmid

   #163459

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. anti-GFP scFv [N86/38] with sortase tag

   Plasmid

   #204419

   Purpose

   Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting GFP (Aequorea victoria)
   

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPC1 MOSPD1

   Plasmid

   #226404

   Purpose

   Expression of human MOSPD1 fused to EGFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   MOSPD1 (MOSPD1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-Tet-On-HDAC1-shRNA2

   Plasmid

   #90241

   Purpose

   Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR-v1-sgGLS_6

   Plasmid

   #163460

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 6 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_016

   Plasmid

   #107143

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   David Root

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-HDAC1-shRNA1

   Plasmid

   #90242

   Purpose

   Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-IRAK2

   Plasmid

   #23412

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   IRAK2 (IRAK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-M-Flag-BAP1-H169Q

    Plasmid

    #125847

    Purpose

    express mutant BAP1-H169Q

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    H169Q

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVHD

    Plasmid

    #61303

    Purpose

    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

    Type

    Empty backbone

    Tags

    HA

    Expression

    Bacterial

    Promoter

    PV10

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HTR2A-DuET

    Plasmid

    #213321

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGlomyc-Klf4

    Plasmid

    #172870

    Purpose

    Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Klf4 (Klf4 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pEBTetD-β-tubulin_SNAP

    Plasmid

    #136832

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    TUBB_SNAP (TUBB Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid

    #90243

    Purpose

    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXRA2

    Plasmid

    #63144

    Purpose

    ADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial and Yeast

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEBTet-SNAP-γ-tubulin

    Plasmid

    #136799

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-TUBG (TUBG1 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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