165,214 results

1. E326K GBA pcDNA3.1

   Plasmid

   #188581

   Purpose

   Ectopic expression E326K GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   E326K

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-PDGFRbeta

   Plasmid

   #136455

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRB

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRbeta (PDGFRB Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-Myc-TEAD1

   Plasmid

   #33109

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

   Insert

   TEAD1 (TEAD1 )
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP15072 - pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA (Alias: CN5072)

   Plasmid

   #230402

   Purpose

   AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-MITF

   Plasmid

   #170695

   Purpose

   doxycycline-inducible overexpression of MITF

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Bcl2

   Plasmid

   #195551

   Purpose

   Expresses Bcl2 in mammalian cells

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Bcl2 (Bcl2 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV enhancer and promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCD-GSDMA-N-tev-C

   Plasmid

   #226231

   Purpose

   Express TEVP-cleavable GSDMA

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   Gasdermin A (Gsdma3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Murine E-cadherin mCherry

   Plasmid

   #71366

   Purpose

   mammalian expression of murine E-caderin with mCherry fusion

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-GST-IRP1

   Plasmid

   #216827

   Purpose

   Expresses human IRP1 with a N-terminal GST-tag in mammalian cells.

   Depositor

   Samie Jaffrey

   Insert

   ACO1 (ACO1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin2 (CUL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS548∙CsR

    Plasmid

    #197859

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Tc resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH0006

    Plasmid

    #135448

    Purpose

    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    dCas9-BFP-KRAB
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a(+)-Histag-mCherry (527)

    Plasmid

    #70719

    Purpose

    Expression vector for mCherry purification

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    mCherry
    

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. BRAF_pLX307

    Plasmid

    #98322

    Purpose

    Lentiviral expression of BRAF

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Expression

    Bacterial

    Promoter

    ApR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKD236-4b

    Plasmid

    #90956

    Purpose

    Expresses ThsS constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    ThsS
    

    Expression

    Bacterial

    Promoter

    Bba_J23104

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZW6 (CK2alpha)

    Plasmid

    #27086

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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