166,778 results

1. pET28a-PACT

   Plasmid

   #50352

   Purpose

   bacterial expression of PACT

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   PACT (PRKRA Human)
   

   Tags

   His and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1-mCherry

   Plasmid

   #102629

   Purpose

   In vitro expression of mCherry from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304-VHL-IRES-Tdtomato

   Plasmid

   #187643

   Purpose

   VHL Expression vector with Tdtomato

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCas9-Venus

   Plasmid

   #70267

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   Cas9
   Venus Reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS and P2A

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/mCherry_pBirA-HA_FKBP8

   Plasmid

   #154457

   Purpose

   expresses mCherry_pBirA-HA_FKBP8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry_pBirA-HA_FKBP8 (FKBP8 Human)
   

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.18 NR0B1-Luc

   Plasmid

   #100983

   Purpose

   NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

   Depositor

   Lee Helman

   Article

   Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

   Insert

   NR0B1 (NR0B1 Human)
   

   Expression

   Mammalian

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH-roGFP2-synORP1

   Plasmid

   #191680

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   rredox(roGFP2-synORP1
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-GRAB_UCN1.0

   Plasmid

   #208667

   Purpose

   Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    UBC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB_VIP1.0

    Plasmid

    #208682

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-His6dNLP

    Plasmid

    #105498

    Purpose

    Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

    Depositor

    James Kadonaga

    Article

    Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

    Insert

    His6dNLP
    

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK340

    Plasmid

    #192639

    Purpose

    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpG has se…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. BCL2alpha-MJK2243

    Plasmid

    #227690

    Purpose

    Expresses human BCL2alpha

    Depositor

    Curt Civin

    Article

    Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

    Insert

    human BCL-2alpha (BCL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2962

    Plasmid

    #144438

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2C2 (NR2C2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG261-His-greenFAST

    Plasmid

    #160349

    Purpose

    Expresses His-tagged greenFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-greenFAST
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-natCX

    Plasmid

    #73893

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    natCX
    

    Expression

    Yeast

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MBP-mmA1

    Plasmid

    #61090

    Purpose

    expression of fusion protein: MBP-hnRNP A1

    Depositor

    Sean Ryder

    Article

    Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

    Insert

    hnRNP A1 (Hnrnpa1 Mouse)
    

    Tags

    H6_MBP

    Expression

    Bacterial

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEBTet-β-tubulin_SNAP

    Plasmid

    #136831

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    TUBB_SNAP (TUBB Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-MTOR-F1888L

    Plasmid

    #69010

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-F1888L (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Phenylalanine 1888 to Leucine

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only