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pCS2-nCas9n Citations (51)

Plasmid Article: Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.
Jao LE, Wente SR, Chen W Proc Natl Acad Sci U S A. 2013 Aug 5.
PubMed Journal

Articles Citing pCS2-nCas9n

Articles
Disruptive CHD8 mutations define a subtype of autism early in development. Bernier R, Golzio C, Xiong B, Stessman HA, Coe BP, Penn O, Witherspoon K, Gerdts J, Baker C, Vulto-van Silfhout AT, Schuurs-Hoeijmakers JH, Fichera M, Bosco P, Buono S, Alberti A, Failla P, Peeters H, Steyaert J, Vissers LE, Francescatto L, Mefford HC, Rosenfeld JA, Bakken T, O'Roak BJ, Pawlus M, Moon R, Shendure J, Amaral DG, Lein E, Rankin J, Romano C, de Vries BB, Katsanis N, Eichler EE. Cell. 2014 Jul 17;158(2):263-76. doi: 10.1016/j.cell.2014.06.017. Epub 2014 Jul 3. PubMed
Efficient homologous recombination-mediated genome engineering in zebrafish using TALE nucleases. Shin J, Chen J, Solnica-Krezel L. Development. 2014 Oct;141(19):3807-18. doi: 10.1242/dev.108019. Epub 2014 Sep 5. PubMed
CRISPR/Cas9-mediated genome modification in the mollusc, Crepidula fornicata. Perry KJ, Henry JQ. Genesis. 2015 Feb;53(2):237-44. doi: 10.1002/dvg.22843. Epub 2015 Jan 9. PubMed
Evaluation of the in vivo safety profiles of Rictor inhibition using a zebrafish model. Cao Y, Jiang L, Zhao L, Zhou X, Wang N, Zhang P, Tang Y, Zhou J. Curr Pharm Des. 2015;21(12):1645-53. PubMed
A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate. Harel I, Benayoun BA, Machado B, Singh PP, Hu CK, Pech MF, Valenzano DR, Zhang E, Sharp SC, Artandi SE, Brunet A. Cell. 2015 Feb 26;160(5):1013-26. doi: 10.1016/j.cell.2015.01.038. Epub 2015 Feb 12. PubMed
The Wnt Co-Receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish. Willems B, Tao S, Yu T, Huysseune A, Witten PE, Winkler C. PLoS One. 2015 Jun 29;10(6):e0131768. doi: 10.1371/journal.pone.0131768. eCollection 2015. PubMed
TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. Gao L, Li D, Ma K, Zhang W, Xu T, Fu C, Jing C, Jia X, Wu S, Sun X, Dong M, Deng M, Chen Y, Zhu W, Peng J, Wan F, Zhou Y, Zon LI, Pan W. PLoS Genet. 2015 Jul 1;11(7):e1005346. doi: 10.1371/journal.pgen.1005346. eCollection 2015 Jul. PubMed
Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Ogawa Y, Shiraki T, Kojima D, Fukada Y. Proc Biol Sci. 2015 Aug 7;282(1812). pii: 20150659. doi: 10.1098/rspb.2015.0659. PubMed
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System. Yin L, Jao LE, Chen W. Methods Mol Biol. 2015;1332:205-17. doi: 10.1007/978-1-4939-2917-7_16. PubMed
Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System. Armstrong GA, Liao M, You Z, Lissouba A, Chen BE, Drapeau P. PLoS One. 2016 Mar 1;11(3):e0150188. doi: 10.1371/journal.pone.0150188. eCollection 2016. PubMed
2C-Cas9: a versatile tool for clonal analysis of gene function. Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F. Genome Res. 2016 Mar 8. PubMed

Associated Plasmids

Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line. Dehler CE, Boudinot P, Martin SA, Collet B. Mar Biotechnol (NY). 2016 May 28. PubMed
Plexins function in epithelial repair in both Drosophila and zebrafish. Yoo SK, Pascoe HG, Pereira T, Kondo S, Jacinto A, Zhang X, Hariharan IK. Nat Commun. 2016 Jul 25;7:12282. doi: 10.1038/ncomms12282. PubMed
Efficient genome engineering approaches for the short-lived African turquoise killifish. Harel I, Valenzano DR, Brunet A. Nat Protoc. 2016 Oct;11(10):2010-2028. doi: 10.1038/nprot.2016.103. Epub 2016 Sep 22. PubMed
Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation. Lau ES, Zhang Z, Qin M, Ge W. Sci Rep. 2016 Nov 23;6:37357. doi: 10.1038/srep37357. PubMed
The Gene Regulatory Network of Lens Induction Is Wired through Meis-Dependent Shadow Enhancers of Pax6. Antosova B, Smolikova J, Klimova L, Lachova J, Bendova M, Kozmikova I, Machon O, Kozmik Z. PLoS Genet. 2016 Dec 5;12(12):e1006441. doi: 10.1371/journal.pgen.1006441. eCollection 2016 Dec. PubMed
Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion. Morris EAR, Bodin S, Delaval B, Comunale F, Georget V, Costa ML, Lutfalla G, Gauthier-Rouviere C. Biol Cell. 2017 May;109(5):210-221. doi: 10.1111/boc.201700001. Epub 2017 Mar 21. PubMed
ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C, Kaufmann U, Karoly Jani P, Lacruz RS, Flockerzi V, Kacskovics I, Prakriya M, Feske S. Nat Commun. 2017 Mar 15;8:14714. doi: 10.1038/ncomms14714. PubMed
Chemical biology reveals CARF as a positive regulator of canonical Wnt signaling by promoting TCF/beta-catenin transcriptional activity. He X, Zhang W, Yan C, Nie F, Li C, Liu X, Fei C, Li S, Song X, Jia Y, Zeng R, Wu D, Pan W, Hao X, Li L. Cell Discov. 2017 Jan 31;3:17003. doi: 10.1038/celldisc.2017.3. eCollection 2017. PubMed
nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Facchinello N, Skobo T, Meneghetti G, Colletti E, Dinarello A, Tiso N, Costa R, Gioacchini G, Carnevali O, Argenton F, Colombo L, Dalla Valle L. Sci Rep. 2017 Jun 29;7(1):4371. doi: 10.1038/s41598-017-04535-6. PubMed
Zygotic vinculin is not essential for embryonic development in zebrafish. Han MKL, van der Krogt GNM, de Rooij J. PLoS One. 2017 Aug 2;12(8):e0182278. doi: 10.1371/journal.pone.0182278. eCollection 2017. PubMed
Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning. Pelliccia JL, Jindal GA, Burdine RD. Elife. 2017 Nov 15;6. pii: 28635. doi: 10.7554/eLife.28635. PubMed
miR-34a Regulates Sperm Motility in Zebrafish. Guo W, Xie B, Xiong S, Liang X, Gui JF, Mei J. Int J Mol Sci. 2017 Dec 10;18(12). pii: ijms18122676. doi: 10.3390/ijms18122676. PubMed
Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1). Kell MJ, Riccio RE, Baumgartner EA, Compton ZJ, Pecorin PJ, Mitchell TA, Topczewski J, LeClair EE. PLoS One. 2018 Jan 2;13(1):e0190353. doi: 10.1371/journal.pone.0190353. eCollection 2018. PubMed
Blocking fatty acid-fueled mROS production within macrophages alleviates acute gouty inflammation. Hall CJ, Sanderson LE, Lawrence LM, Pool B, van der Kroef M, Ashimbayeva E, Britto D, Harper JL, Lieschke GJ, Astin JW, Crosier KE, Dalbeth N, Crosier PS. J Clin Invest. 2018 May 1;128(5):1752-1771. doi: 10.1172/JCI94584. Epub 2018 Mar 26. PubMed
Whole-organism clone tracing using single-cell sequencing. Alemany A, Florescu M, Baron CS, Peterson-Maduro J, van Oudenaarden A. Nature. 2018 Apr 5;556(7699):108-112. doi: 10.1038/nature25969. Epub 2018 Mar 28. PubMed
Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus). Chen J, Wang W, Tian Z, Dong Y, Dong T, Zhu H, Zhu Z, Hu H, Hu W. Front Genet. 2018 Apr 6;9:117. doi: 10.3389/fgene.2018.00117. eCollection 2018. PubMed
Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype. de Vos IJHM, Tao EY, Ong SLM, Goggi JL, Scerri T, Wilson GR, Low CGM, Wong ASW, Grussu D, Stegmann APA, van Geel M, Janssen R, Amor DJ, Bahlo M, Dunn NR, Carney TJ, Lockhart PJ, Coull BJ, van Steensel MAM. Hum Mol Genet. 2018 Aug 15;27(16):2775-2788. doi: 10.1093/hmg/ddy168. PubMed
Failed Progenitor Specification Underlies the Cardiopharyngeal Phenotypes in a Zebrafish Model of 22q11.2 Deletion Syndrome. Guner-Ataman B, Gonzalez-Rosa JM, Shah HN, Butty VL, Jeffrey S, Abrial M, Boyer LA, Burns CG, Burns CE. Cell Rep. 2018 Jul 31;24(5):1342-1354.e5. doi: 10.1016/j.celrep.2018.06.117. PubMed
RAS at the Golgi antagonizes malignant transformation through PTPRkappa-mediated inhibition of ERK activation. Casar B, Badrock AP, Jimenez I, Arozarena I, Colon-Bolea P, Lorenzo-Martin LF, Barinaga-Rementeria I, Barriuso J, Cappitelli V, Donoghue DJ, Bustelo XR, Hurlstone A, Crespo P. Nat Commun. 2018 Sep 5;9(1):3595. doi: 10.1038/s41467-018-05941-8. PubMed
MiR-202 controls female fecundity by regulating medaka oogenesis. Gay S, Bugeon J, Bouchareb A, Henry L, Delahaye C, Legeai F, Montfort J, Le Cam A, Siegel A, Bobe J, Thermes V. PLoS Genet. 2018 Sep 10;14(9):e1007593. doi: 10.1371/journal.pgen.1007593. eCollection 2018 Sep. PubMed
Zebrafish expression reporters and mutants reveal that the IgSF cell adhesion molecule Dscamb is required for feeding and survival. Julien DP, Chan AW, Barrios J, Mathiaparanam J, Douglass A, Wolman MA, Sagasti A. J Neurogenet. 2018 Dec;32(4):336-352. doi: 10.1080/01677063.2018.1493479. Epub 2018 Sep 11. PubMed
Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders. Tessadori F, Roessler HI, Savelberg SMC, Chocron S, Kamel SM, Duran KJ, van Haelst MM, van Haaften G, Bakkers J. Dis Model Mech. 2018 Oct 18;11(10). pii: 11/10/dmm035469. doi: 10.1242/dmm.035469. PubMed
ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm. Gould RA, Aziz H, Woods CE, Seman-Senderos MA, Sparks E, Preuss C, Wunnemann F, Bedja D, Moats CR, McClymont SA, Rose R, Sobreira N, Ling H, MacCarrick G, Kumar AA, Luyckx I, Cannaerts E, Verstraeten A, Bjork HM, Lehsau AC, Jaskula-Ranga V, Lauridsen H, Shah AA, Bennett CL, Ellinor PT, Lin H, Isselbacher EM, Lino Cardenas CL, Butcher JT, Hughes GC, Lindsay ME, Mertens L, Franco-Cereceda A, Verhagen JMA, Wessels M, Mohamed SA, Eriksson P, Mital S, Van Laer L, Loeys BL, Andelfinger G, McCallion AS, Dietz HC. Nat Genet. 2019 Jan;51(1):42-50. doi: 10.1038/s41588-018-0265-y. Epub 2018 Nov 19. PubMed
The epg5 knockout zebrafish line: a model to study Vici syndrome. Meneghetti G, Skobo T, Chrisam M, Facchinello N, Fontana CM, Bellesso S, Sabatelli P, Raggi F, Cecconi F, Bonaldo P, Dalla Valle L. Autophagy. 2019 Aug;15(8):1438-1454. doi: 10.1080/15548627.2019.1586247. Epub 2019 Mar 17. PubMed
The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone B. Castaneda Cortes DC, Padilla LFA, Langlois VS, Somoza GM, Fernandino JI. Development. 2019 Apr 23;146(8). pii: dev.172866. doi: 10.1242/dev.172866. PubMed
NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Princely Abudu Y, Pankiv S, Mathai BJ, Hakon Lystad A, Bindesboll C, Brenne HB, Yoke Wui Ng M, Thiede B, Yamamoto A, Mutugi Nthiga T, Lamark T, Esguerra CV, Johansen T, Simonsen A. Dev Cell. 2019 May 20;49(4):509-525.e12. doi: 10.1016/j.devcel.2019.03.013. Epub 2019 Apr 11. PubMed
The development of CRISPR for a mollusc establishes the formin Lsdia1 as the long-sought gene for snail dextral/sinistral coiling. Abe M, Kuroda R. Development. 2019 May 14;146(9). pii: dev.175976. doi: 10.1242/dev.175976. PubMed
EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells. Grainger S, Nguyen N, Richter J, Setayesh J, Lonquich B, Oon CH, Wozniak JM, Barahona R, Kamei CN, Houston J, Carrillo-Terrazas M, Drummond IA, Gonzalez D, Willert K, Traver D. Nat Cell Biol. 2019 Jun;21(6):721-730. doi: 10.1038/s41556-019-0330-5. Epub 2019 May 20. PubMed
yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets. Vazquez-Marin J, Gutierrez-Triana JA, Almuedo-Castillo M, Buono L, Gomez-Skarmeta JL, Mateo JL, Wittbrodt J, Martinez-Morales JR. Development. 2019 Jun 21;146(13). pii: dev.173286. doi: 10.1242/dev.173286. PubMed
Dysregulation in the Brain Protein Profile of Zebrafish Lacking the Parkinson's Disease-Related Protein DJ-1. Edson AJ, Hushagen HA, Froyset AK, Elda I, Khan EA, Di Stefano A, Fladmark KE. Mol Neurobiol. 2019 Jun 19. pii: 10.1007/s12035-019-01667-w. doi: 10.1007/s12035-019-01667-w. PubMed
The Novel Small Molecule TRVA242 Stabilizes Neuromuscular Junction Defects in Multiple Animal Models of Amyotrophic Lateral Sclerosis. Bose P, Tremblay E, Maois C, Narasimhan V, Armstrong GAB, Liao M, Parker JA, Robitaille R, Wen XY, Barden C, Drapeau P. Neurotherapeutics. 2019 Oct;16(4):1149-1166. doi: 10.1007/s13311-019-00765-w. PubMed
ECM alterations in Fndc3a (Fibronectin Domain Containing Protein 3A) deficient zebrafish cause temporal fin development and regeneration defects. Liedtke D, Orth M, Meissler M, Geuer S, Knaup S, Koblitz I, Klopocki E. Sci Rep. 2019 Sep 16;9(1):13383. doi: 10.1038/s41598-019-50055-w. PubMed
Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Oprisoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG. Cell Rep. 2019 Oct 29;29(5):1082-1098.e10. doi: 10.1016/j.celrep.2019.09.033. PubMed
Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish. Idigo NJ, Soares DC, Abbott CM. Biosci Rep. 2020 Jan 31;40(1). pii: 221899. doi: 10.1042/BSR20194191. PubMed
NaV1.1 and NaV1.6 selective compounds reduce the behavior phenotype and epileptiform activity in a novel zebrafish model for Dravet Syndrome. Weuring WJ, Singh S, Volkers L, Rook MB, van 't Slot RH, Bosma M, Inserra M, Vetter I, Verhoeven-Duif NM, Braun KPJ, Rivara M, Koeleman BPC. PLoS One. 2020 Mar 5;15(3):e0219106. doi: 10.1371/journal.pone.0219106. eCollection 2020. PubMed
Defining endogenous barcoding sites for CRISPR/Cas9-based cell lineage tracing in zebrafish. Ye C, Chen Z, Liu Z, Wang F, He X. J Genet Genomics. 2020 Feb 20;47(2):85-91. doi: 10.1016/j.jgg.2019.11.012. Epub 2020 Feb 7. PubMed
Zebrafish ambra1a and ambra1b Silencing Affect Heart Development. Meneghetti G, Skobo T, Chrisam M, Fontana CM, Facchinello N, Nazio F, Cecconi F, Bonaldo P, Dalla Valle L. Zebrafish. 2020 Apr 22. doi: 10.1089/zeb.2020.1860. PubMed
Barcode sequence could be a good target for developing a species-specific anti-parasite agent based on CRISPR-Cas9. Pan Y, Sun Y, Wang Y, Zhang Z. FASEB J. 2020 May 31. doi: 10.1096/fj.202000118RR. PubMed
Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis. Bottiglione F, Dee CT, Lea R, Zeef LAH, Badrock AP, Wane M, Bugeon L, Dallman MJ, Allen JE, Hurlstone AFL. J Immunol. 2020 Aug 15;205(4):994-1008. doi: 10.4049/jimmunol.2000372. Epub 2020 Jul 8. PubMed
suz12 inactivation in p53- and nf1-deficient zebrafish accelerates the onset of malignant peripheral nerve sheath tumors and expands the spectrum of tumor types. Oppel F, Ki DH, Zimmerman MW, Ross KN, Tao T, Shi H, He S, Aster JC, Look AT. Dis Model Mech. 2020 Aug 27;13(8). pii: dmm.042341. doi: 10.1242/dmm.042341. PubMed

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