pCS2+ heiCas9
(Plasmid #186929)

• Purpose: For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
• Depositing Lab: Joachim Wittbrodt
• Publication: Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4065
• Total vector size (bp): 8291
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: myc-flexible linker-oNLS-Cas9-oNLS
• Alt name: heiCas9
• Species: Synthetic; S. pyogenes
• Insert Size (bp): 4212
• Promoter: sp6
• Tags / Fusion Proteins:
  • myc-flexible linker-optimizedNLS (N terminal on insert)
  • optimizedNLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: sp6
• 3′ sequencing primer: pCEP-rev

Resource Information

• Supplemental Documents:
  • pCS2-heiCas9.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry