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pCS2+ heiCas9
(Plasmid #186929)


Item Catalog # Description Quantity Price (USD)
Plasmid 186929 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4065
  • Total vector size (bp) 8291
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    myc-flexible linker-oNLS-Cas9-oNLS
  • Alt name
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
  • Promoter sp6
  • Tags / Fusion Proteins
    • myc-flexible linker-optimizedNLS (N terminal on insert)
    • optimizedNLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer sp6
  • 3′ sequencing primer pCEP-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ heiCas9 was a gift from Joachim Wittbrodt (Addgene plasmid # 186929 ; ; RRID:Addgene_186929)
  • For your References section:

    Boosting targeted genome editing using the hei-tag. Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, Medert R, Welz B, Freichel M, Wittbrodt J. Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558. 10.7554/eLife.70558 PubMed 35333175