pCS2+ heiCas9
(Plasmid
#186929)
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PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
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Depositing Lab
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Depositing OrganizationUniversitaet Heidelberg
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 186929-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 186929-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4065
- Total vector size (bp) 8291
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemyc-flexible linker-oNLS-Cas9-oNLS
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Alt nameheiCas9
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SpeciesSynthetic; S. pyogenes
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Insert Size (bp)4212
- Promoter sp6
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Tags
/ Fusion Proteins
- myc-flexible linker-optimizedNLS (N terminal on insert)
- optimizedNLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer sp6
- 3′ sequencing primer pCEP-rev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 186929-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 186929-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ heiCas9 was a gift from Joachim Wittbrodt (Addgene plasmid # 186929 ; http://n2t.net/addgene:186929 ; RRID:Addgene_186929) -
For your References section:
Boosting targeted genome editing using the hei-tag. Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, Medert R, Welz B, Freichel M, Wittbrodt J. Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558. 10.7554/eLife.70558 PubMed 35333175
