166,716 results

1. pcDNA3.1-Flag-RBM24

   Plasmid

   #208052

   Purpose

   Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000379052) coding region (RBM24 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA7-SacB

   Plasmid

   #79968

   Purpose

   TM-MAGE strain

   Depositor

   Morten Sommer

   Article

   Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

   Inserts

   Lambda Red recombinase beta subunit
   DNA adenine methylase
   Levansucrase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2-Ub-G76V-GFP

   Plasmid

   #11954

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-G76V-GFP
   

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Expression

   Bacterial

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T1_4xUb

   Plasmid

   #199779

   Purpose

   Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   4X Ubiquitin in Tandem
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSHDY

   Plasmid

   #137661

   Purpose

   Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ220 (AsCpf1)

   Plasmid

   #86208

   Purpose

   AsCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   AsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   5' and 3' NLS

   Expression

   Plant

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSRK2721

   Plasmid

   #78572

   Purpose

   Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   Gateway cloning cassette
   

   Expression

   Bacterial

   Available Since

   Sept. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLC20A1_STOP

    Plasmid

    #161176

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC20A1 (SLC20A1 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pRK5_mCherry-GFP-nb-KS

    Plasmid

    #238260

    Purpose

    For overexpression of mCherry-GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-GFP-nb-KS
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFW2100

    Plasmid

    #224212

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV-EF1aGFP2AKAT7(E508Q)-W

    Plasmid

    #159293

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 E508Q
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

    Promoter

    human EF1a promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFGC5941-PacI

    Plasmid

    #44182

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLib6.4B-Aalb_743

    Plasmid

    #176676

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    FOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ptc1-YFP

    Plasmid

    #58456

    Purpose

    expression of mouse Ptc1-YFP

    Depositor

    Matthew Scott

    Article

    Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

    Insert

    Ptc1 (Ptch1 Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

18. miniCMV eGFP hPGK BSD

    Plasmid

    #188519

    Purpose

    Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-T-GECO1

    Plasmid

    #191616

    Purpose

    Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

    Depositor

    Robert Campbell

    Article

    Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

    Insert

    T-GECO1
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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