165,214 results

1. pIIIA/MS2-1

   Plasmid

   #220631

   Purpose

   For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-T-rnVAPA-1-219

   Plasmid

   #13395

   Depositor

   Axel Brunger

   Article

   Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

   Insert

   VAP-A (Vapa Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pG1C

   Plasmid

   #71740

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Bacterial

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-GLIS1

   Plasmid

   #30166

   Purpose

   Retroviral expression of non-canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-shRNA

   Plasmid

   #82697

   Purpose

   For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Type

   Empty backbone

   Use

   RNAi

   Promoter

   hPGK, U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 TMEM192-Flag-FKBP

   Plasmid

   #112743

   Purpose

   express TMEM192-Flag-FKBP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   TMEM192-Flag-FKBP (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag, FKBP

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HiBiT-FZD7

   Plasmid

   #195845

   Purpose

   Expresses human FZD7 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD7 (FZD7 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNos-PE2-attB

   Plasmid

   #149549

   Purpose

   Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   nanos (nos)

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hSNCA-NE

   Plasmid

   #102361

   Purpose

   Overexpression of synuclein alpha in mammalian cells

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Tags

   NE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-eGFP-uTEV3

    Plasmid

    #234320

    Purpose

    Mammalian expression of uTEV3 tagged with an eGFP marker

    Depositor

    Mateo Sánchez

    Article

    Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

    Insert

    eGFP-uTEV3
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Mutations respective to the wild type TEV: S219V …

    Promoter

    CMV

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQ79

    Plasmid

    #175064

    Purpose

    expresses mutant of the huntingtin exon1 in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    polyQ79 (HTT Human)
    

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKD239-1g-2

    Plasmid

    #90959

    Purpose

    Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23100, Bba_J23109, and PttrB185-269

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMF1706

    Plasmid

    #125582

    Purpose

    Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

    Depositor

    Marc Fransen

    Article

    Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

    Insert

    po-roGFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC4-RhE-FRB-Fis1

    Plasmid

    #68056

    Purpose

    express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

    Insert

    Fis1 (FIS1 Human)
    

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    Fis1 tail (aa 93-152)

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVITRO1-mCardinal-CAXX

    Plasmid

    #184451

    Purpose

    Plasma membrane-targeted mCardinal expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCardinal-CAAX
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV2_HLF-ZP

    Plasmid

    #175035

    Purpose

    HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A Puro
    

    Use

    AAV

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-NLyn-Venus-Zdk1

    Plasmid

    #81042

    Purpose

    mammalian expression of plasma-membrane anchored Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, NLyn, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6p-1-UFBP1

    Plasmid

    #204558

    Purpose

    Expresses GST-tagged UFBP1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFBP1 (DDRGK1 Human)
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHelper_NoMutL_V2_gentR

    Plasmid

    #205293

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-BsaI

    Plasmid

    #54340

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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