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Cre Reporter

Plasmid

#62732

Purpose

Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

LoxP-DsRED-STOP-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Tags

LoxP sites

Expression

Mammalian

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Publication

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pLenti CMV GFP DEST (736-1)

Plasmid

#19732

Purpose

3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCX-OKS-2A

Plasmid

#19771

Purpose

Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

Depositor

Shinya Yamanaka

Publication

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)

Expression

Mammalian

Mutation

Three genes are connected with the foot-and-mouth…

Available since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Publication

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Publication

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

19 Articles

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pEGFP-Q74

Plasmid

#40262

Depositor

David Rubinsztein

Publication

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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pLX302

Plasmid

#25896

Purpose

3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5

Expression

Mammalian

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

55 Articles

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Flag-TurboID

Plasmid

#124646

Purpose

Expresses Flag-TurboID fusion for proximity labeling

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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mito-V5-APEX2

Plasmid

#72480

Purpose

Expresses APEX2 in mitochondria

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

V5

Expression

Mammalian

Promoter

CMV promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Publication

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pcDNA3-EGFP-RhoA-wt

Plasmid

#12965

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pcDNA3.1-HA

Plasmid

#128034

Purpose

Mammalian expression vector with N terminal HA tag

Depositor

Oskar Laur

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Steven Carr, Samuel Myers

Publication

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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PX551

Plasmid

#60957

Purpose

pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

Depositor

Feng Zhang

Publication

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Insert

SpCas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

pMecp2

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pMSCV-IRES-GFP II (pMIG II)

Plasmid

#52107

Purpose

a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Publication

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

64 Articles

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