161,252 results

1. pVL1392 TMEM97

   Plasmid

   #157748

   Purpose

   Baculovirus transfer vectors to create a virus expressing human TMEM97

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBSFI-myr-akt-T308D/S473D

   Plasmid

   #49192

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308D; S473D

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-mEGFP

   Plasmid

   #127813

   Purpose

   Hetero-dimer mCherry-mEGFP expression vector

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   mCherry-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1330

   Plasmid

   #143950

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2C (TFAP2C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgTP53_3

   Plasmid

   #78164

   Purpose

   sgTP53

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pWZL Neo Myr Flag PTK2

   Plasmid

   #20610

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   PTK2 (PTK2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBB-XIAPmut

   Plasmid

   #11559

   Depositor

   Jon Ashwell

   Article

   Yang et al Science 2000 May 5;288(5467):874-7.

   Insert

   XIAPmut (XIAP Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed his 467 to Alanine

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

8. TFORF1317

   Plasmid

   #143788

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RB1CC1 (RB1CC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD-iPASnFR-H

   Plasmid

   #225237

   Purpose

   Bacterial expression of iPASnFR-H

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbS8a-RFP

    Plasmid

    #35274

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. MRGPRD-DuET

    Plasmid

    #213340

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRD (MRGPRD Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag C/EBPb LAP

    Plasmid

    #66979

    Purpose

    Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP beta (Cebpb Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-Flag-NPM1c

    Plasmid

    #131813

    Purpose

    Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ADAMTS5_pCSdest

    Plasmid

    #53819

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    ADAMTS5 (ADAMTS5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231566

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Twin-Strep(R)-Tev-GSGS-Ubiquitin

    Plasmid

    #186803

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Twin-Strep(R)-Tev-GSGS-Ubiquitin
    

    Use

    Tags

    GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

    Expression

    Bacterial

    Mutation

    Strep tag Lysine's mutated to Arginine

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-GCaMP6s-WPRE-pGHpA

    Plasmid

    #67526

    Purpose

    "Fluorescent reporter for calcium imaging"

    Depositor

    Botond Roska

    Article

    Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. N1-Mito-pHCountdown

    Plasmid

    #182303

    Purpose

    mammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    mitochondria targeted pH countdown
    

    Use

    Tags

    COX8A mitochondria targeting sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 MLK1

    Plasmid

    #60540

    Purpose

    Donor vector for human MLK1

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only