165,256 results

1. TR-Csy4-R115A/R119A

   Plasmid

   #80696

   Purpose

   Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-R115A/R119A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Arg115 and Arg119 both mutated to Ala

   Promoter

   CBA

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-RET

   Plasmid

   #116787

   Purpose

   Lentiviral expression of RET

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   RET (RET Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-U6-sgRNA-CMV-puro

   Plasmid

   #169450

   Purpose

   backbone for sgRNA expression

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   sgRNA
   

   Use

   Lentiviral

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE smFP-V5 donor (all-in-one) ORF1

   Plasmid

   #200387

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hNTo1-qgRNA-pYJA5

   Plasmid

   #217781

   Purpose

   Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. hNTo2-qgRNA-pYJA5

   Plasmid

   #217782

   Purpose

   Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Hhe_Cas13a_WT

   Plasmid

   #91871

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   HheCas13a (HheC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR223 CD147, open

   Plasmid

   #149720

   Purpose

   for Gateway cloning of CD147 into destination vector

   Depositor

   Anne-Claude Gingras

   Insert

   CD147 (BSG Human)
   

   Use

   Entry vector

   Mutation

   wt, without stop codon

   Available Since

   June 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET15b-17X-PTDH

    Plasmid

    #166786

    Purpose

    Phosphite dehydrogenase for NAD(P)H regeneration

    Depositor

    Wilfred van der Donk

    Article

    Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

    Insert

    Phosphite dehydroenase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A196R, T201S, A328T, E352N, and C356D- please see…

    Promoter

    T7

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. non-targeting control gRNA (BRDN0001487130)

    Plasmid

    #80262

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid

    #215119

    Purpose

    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Inserts

    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-tag, HA-tag, and SS (STREP-STREP)

    Expression

    Mammalian

    Promoter

    IRES and TREG3

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX GFP-HA

    Plasmid

    #229501

    Purpose

    Control HA-tagged protein for immunoprecipitation experiments.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TetON-CD4

    Plasmid

    #217537

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    CD4 receptor (CD4 Human)
    

    Use

    AAV

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    TetOperon (TetO)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SQ171fg/pRibo-T

    Plasmid

    #69343

    Purpose

    SQ171 fast growing strain supported by pRibo-T plasmid

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    Ribo-T
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZE27GFP3

    Plasmid

    #75456

    Purpose

    Plasmid for constitutive expression of gfp-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp-pdt#3
    

    Expression

    Bacterial

    Promoter

    placIq promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEB2-mNeptune2.5

    Plasmid

    #104013

    Purpose

    Plasmid encoding mNeptune2.5

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mNeptune2.5
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (MSKGEE LIKENM… M11…

    Promoter

    proC

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE:iRFP670-EFS:Cre-2A-GFP

    Plasmid

    #68477

    Purpose

    Inducible lentivirus expressing iRFP670, Cre, and GFP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    iRFP670
    Cre-2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-TEX264-GFP

    Plasmid

    #201925

    Purpose

    For lentiviral transduction of TEX264-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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