Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Citations (21)

Originally described in: Controlled gene expression in primary Lgr5 organoid cultures.
Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802.
PubMed Journal

Articles Citing pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Articles
P2X-selective purinergic antagonists are strong inhibitors of HIV-1 fusion during both cell-to-cell and cell-free infection. Swartz TH, Esposito AM, Durham ND, Hartmann BM, Chen BK. J Virol. 2014 Oct;88(19):11504-15. doi: 10.1128/JVI.01158-14. Epub 2014 Jul 16. PubMed
A video protocol of retroviral infection in primary intestinal organoid culture. Andersson-Rolf A, Fink J, Mustata RC, Koo BK. J Vis Exp. 2014 Aug 11;(90):e51765. doi: 10.3791/51765. PubMed
Ascl2 acts as an R-spondin/Wnt-responsive switch to control stemness in intestinal crypts. Schuijers J, Junker JP, Mokry M, Hatzis P, Koo BK, Sasselli V, van der Flier LG, Cuppen E, van Oudenaarden A, Clevers H. Cell Stem Cell. 2015 Feb 5;16(2):158-70. doi: 10.1016/j.stem.2014.12.006. Epub 2015 Jan 22. PubMed
Generation of a variety of stable Influenza A reporter viruses by genetic engineering of the NS gene segment. Reuther P, Gopfert K, Dudek AH, Heiner M, Herold S, Schwemmle M. Sci Rep. 2015 Jun 12;5:11346. doi: 10.1038/srep11346. PubMed
Syndecan-4 Is a Receptor for Clathrin-Mediated Endocytosis of Arginine-Rich Cell-Penetrating Peptides. Kawaguchi Y, Takeuchi T, Kuwata K, Chiba J, Hatanaka Y, Nakase I, Futaki S. Bioconjug Chem. 2016 Apr 20;27(4):1119-30. doi: 10.1021/acs.bioconjchem.6b00082. Epub 2016 Apr 5. PubMed
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Broutier L, Andersson-Rolf A, Hindley CJ, Boj SF, Clevers H, Koo BK, Huch M. Nat Protoc. 2016 Sep;11(9):1724-43. doi: 10.1038/nprot.2016.097. Epub 2016 Aug 25. PubMed
Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo. Roh-Johnson M, Shah AN, Stonick JA, Poudel KR, Kargl J, Yang GH, di Martino J, Hernandez RE, Gast CE, Zarour LR, Antoku S, Houghton AM, Bravo-Cordero JJ, Wong MH, Condeelis J, Moens CB. Dev Cell. 2017 Dec 4;43(5):549-562.e6. doi: 10.1016/j.devcel.2017.11.003. PubMed
Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B-cell programming. Li R, Cauchy P, Ramamoorthy S, Boller S, Chavez L, Grosschedl R. Genes Dev. 2018 Jan 15;32(2):96-111. doi: 10.1101/gad.309583.117. Epub 2018 Feb 9. PubMed
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. Esposito AM, Soare AY, Patel F, Satija N, Chen BK, Swartz TH. J Vis Exp. 2018 Aug 14;(138). doi: 10.3791/58074. PubMed
Cell fusion potentiates tumor heterogeneity and reveals circulating hybrid cells that correlate with stage and survival. Gast CE, Silk AD, Zarour L, Riegler L, Burkhart JG, Gustafson KT, Parappilly MS, Roh-Johnson M, Goodman JR, Olson B, Schmidt M, Swain JR, Davies PS, Shasthri V, Iizuka S, Flynn P, Watson S, Korkola J, Courtneidge SA, Fischer JM, Jaboin J, Billingsley KG, Lopez CD, Burchard J, Gray J, Coussens LM, Sheppard BC, Wong MH. Sci Adv. 2018 Sep 12;4(9):eaat7828. doi: 10.1126/sciadv.aat7828. eCollection 2018 Sep. PubMed
A microbial-based cancer vaccine for induction of EGFRvIII-specific CD8+ T cells and anti-tumor immunity. Zebertavage L, Bambina S, Shugart J, Alice A, Zens KD, Lauer P, Hanson B, Gough MJ, Crittenden MR, Bahjat KS. PLoS One. 2019 Jan 2;14(1):e0209153. doi: 10.1371/journal.pone.0209153. eCollection 2019. PubMed
p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Carugo A, Minelli R, Sapio L, Soeung M, Carbone F, Robinson FS, Tepper J, Chen Z, Lovisa S, Svelto M, Amin S, Srinivasan S, Del Poggetto E, Loponte S, Puca F, Dey P, Malouf GG, Su X, Li L, Lopez-Terrada D, Rakheja D, Lazar AJ, Netto GJ, Rao P, Sgambato A, Maitra A, Tripathi DN, Walker CL, Karam JA, Heffernan TP, Viale A, Roberts CWM, Msaouel P, Tannir NM, Draetta GF, Genovese G. Cancer Cell. 2019 Feb 11;35(2):204-220.e9. doi: 10.1016/j.ccell.2019.01.006. PubMed
Human melanoma brain metastases cell line MUG-Mel1, isolated clones and their detailed characterization. Heitzer E, Groenewoud A, Meditz K, Lohberger B, Liegl-Atzwanger B, Prokesch A, Kashofer K, Behrens D, Haybaeck J, Kolb-Lenz D, Koefeler H, Riedl S, Schaider H, Fischer C, Snaar-Jagalska BE, de'Jong D, Szuhai K, Zweytick D, Rinner B. Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.1038/s41598-019-40570-1. PubMed

Associated Plasmids

Chemical suppression of specific C-C chemokine signaling pathways enhances cardiac reprogramming. Guo Y, Lei I, Tian S, Gao W, Hacer K, Li Y, Wang S, Liu L, Wang Z. J Biol Chem. 2019 Jun 7;294(23):9134-9146. doi: 10.1074/jbc.RA118.006000. Epub 2019 Apr 25. PubMed
Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis. Godet I, Shin YJ, Ju JA, Ye IC, Wang G, Gilkes DM. Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1. PubMed

Associated Plasmids

Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia. Braun TP, Okhovat M, Coblentz C, Carratt SA, Foley A, Schonrock Z, Smith BM, Nevonen K, Davis B, Garcia B, LaTocha D, Weeder BR, Grzadkowski MR, Estabrook JC, Manning HG, Watanabe-Smith K, Jeng S, Smith JL, Leonti AR, Ries RE, McWeeney S, Di Genua C, Drissen R, Nerlov C, Meshinchi S, Carbone L, Druker BJ, Maxson JE. Nat Commun. 2019 Nov 29;10(1):5455. doi: 10.1038/s41467-019-13364-2. PubMed
A single-embryo, single-cell time-resolved model for mouse gastrulation. Mittnenzweig M, Mayshar Y, Cheng S, Ben-Yair R, Hadas R, Rais Y, Chomsky E, Reines N, Uzonyi A, Lumerman L, Lifshitz A, Mukamel Z, Orenbuch AH, Tanay A, Stelzer Y. Cell. 2021 May 27;184(11):2825-2842.e22. doi: 10.1016/j.cell.2021.04.004. Epub 2021 Apr 30. PubMed
A persistent invasive phenotype in post-hypoxic tumor cells is revealed by fate mapping and computational modeling. Rocha HL, Godet I, Kurtoglu F, Metzcar J, Konstantinopoulos K, Bhoyar S, Gilkes DM, Macklin P. iScience. 2021 Aug 4;24(9):102935. doi: 10.1016/j.isci.2021.102935. eCollection 2021 Sep 24. PubMed
The intrinsic and extrinsic effects of TET proteins during gastrulation. Cheng S, Mittnenzweig M, Mayshar Y, Lifshitz A, Dunjic M, Rais Y, Ben-Yair R, Gehrs S, Chomsky E, Mukamel Z, Rubinstein H, Schlereth K, Reines N, Orenbuch AH, Tanay A, Stelzer Y. Cell. 2022 Aug 18;185(17):3169-3185.e20. doi: 10.1016/j.cell.2022.06.049. Epub 2022 Jul 30. PubMed
A Rapid, Functional sgRNA Screening Method for Generating Murine RET and NTRK1 Fusion Oncogenes. Schubert L, Le AT, Hinz TK, Navarro A, Nelson-Taylor SK, Nemenoff RA, Heasley LE, Doebele RC. bioRxiv. 2023 Apr 6:2023.04.06.535912. doi: 10.1101/2023.04.06.535912. Preprint. PubMed
A functional sgRNA-CRISPR screening method for generating murine RET and NTRK1 rearranged oncogenes. Schubert L, Le AT, Hinz TK, Navarro AC, Nelson-Taylor SK, Nemenoff RA, Heasley LE, Doebele RC. Biol Open. 2023 Aug 15;12(8):bio059994. doi: 10.1242/bio.059994. Epub 2023 Aug 15. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.