165,256 results

1. pHAGE-TEX264ΔLIR-GFP

   Plasmid

   #201926

   Purpose

   For lentiviral transduction of TEX264ΔLIR-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Lentiviral

   Mutation

   F273A

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. sfGFP-Peroxisomes-2

   Plasmid

   #54601

   Purpose

   Localization: Peroxisomes, Excitation: 485, Emission: 507

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   Peroximal Targeting Signal 1 (PTS1) and sfGFP

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCWXPGR-pTF-Kir2.1

   Plasmid

   #114284

   Purpose

   TET-inducible expression of the Kir2.1 protein

   Depositor

   Patrick Salmon

   Insert

   Kir2.1 (KCNJ2 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCKOv3

   Plasmid

   #209046

   Purpose

   Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LaM8-GG15-irFP

   Plasmid

   #159591

   Purpose

   Opto-Nanobody

   Depositor

   Jared Toettcher, Jose Avalos

   Article

   Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

   Insert

   LaM8 with AsLOV2 at GG15
   

   Use

   Lentiviral

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TR-Csy4-H29A

   Plasmid

   #80602

   Purpose

   Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-H29A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   His29 mutated to Ala

   Promoter

   CBA

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GNb-mCherry

   Plasmid

   #128769

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   GNb-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH7WG2B-OsMIR390-B/c

   Plasmid

   #61465

   Purpose

   Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   OsUBI

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG20_mCherry_Hyg

    Plasmid

    #159701

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Plant

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZsGreen1-H2B-6

    Plasmid

    #56514

    Purpose

    Localization: Nucleus/Histones, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    ZsGreen1

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT/T0-Flag-CIP2A

    Plasmid

    #222623

    Purpose

    mammalian expression vector of Flag-tagged CIP2A

    Depositor

    Daniel Durocher

    Article

    Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

    Insert

    CIP2A (CIP2A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDONR223-EPHA1

    Plasmid

    #23929

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EPHA1 (EPHA1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRRlsinPGK_dectintagBFP2_WPRE

    Plasmid

    #91786

    Purpose

    Constitutive expression of mTagBFP2-Dectin1A-N-10 using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Dectin1A (CLEC7A Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p667-UBC-PKMYT1-V5-miniTurbo_IDG-K

    Plasmid

    #135246

    Purpose

    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    PKMYT1-V5-miniTurbo (PKMYT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. GNb-pHuji

    Plasmid

    #128776

    Purpose

    Mammalian expression

    Depositor

    Colin Taylor

    Article

    Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

    Insert

    GNb-pHuji
    

    Expression

    Mammalian

    Available Since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

    Plasmid

    #159809

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-G12-CheRiff-T2A-mCherry

    Plasmid

    #170275

    Purpose

    CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    CheRiff-T2A-mCherry
    

    Use

    AAV

    Promoter

    11xUAS with TATA-box minimal promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. NES-EGFP-cPHx1

    Plasmid

    #116853

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 )
    

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Amino acids 169.329

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER21-puro_TAOK1

    Plasmid

    #209777

    Purpose

    doxycycline-inducible expression of TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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