161,256 results

1. pcDNA3.1+ Flag-RAP2A

   Plasmid

   #128099

   Purpose

   Expresses RAP2A in mammalian cells in a G418-selectable plasmid

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRVV578

   Plasmid

   #108279

   Purpose

   RMCE vector containing inverted PhiC31 attB sites

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attB sites
   

   Use

   CRISPR; Rmce

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

   Plasmid

   #159539

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19_GZG

   Plasmid

   #117226

   Purpose

   shble flanked by an endogenous GAPDH promoter and terminator system

   Depositor

   Jackie Collier

   Insert

   shble
   

   Use

   Thraustochytrid expression

   Tags

   Expression

   Mutation

   Promoter

   GAPDH

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbS8k-RFP

   Plasmid

   #35276

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAG-NeuroD6iresGFP

   Plasmid

   #45027

   Depositor

   Connie Cepko

   Article

   Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

   Insert

   NeuroD6 (Neurod6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSETb-rsGreen0.7

   Plasmid

   #78176

   Purpose

   Expresses rsGreen0.7 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen0.7
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRM15

   Plasmid

   #163694

   Purpose

   hsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB:mScarlet-P2A-H2B:miRFP670 (HELB, H2BC11 Human)
   

   Use

   Zebrafish expression

   Tags

   mScarlet and miRFP670

   Expression

   Mutation

   Promoter

   HSP70

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SP6-VEE-OKS-iG

   Plasmid

   #58975

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   GLIS (GLIS1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5E26 (PANK2)

    Plasmid

    #79516

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK2 (PANK2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAd-DsRed

    Plasmid

    #17515

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    pAd-DsRed
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

12. hCdh1 (1-125)

    Plasmid

    #8508

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hCdh1 (1-125) (FZR1 Human)
    

    Use

    Retroviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    Cdh1 N-terminus. Dominant Negative mutant.

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

13. SMC2 CRISPR

    Plasmid

    #140649

    Purpose

    SMC2 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    SMC2 (SMC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTBRII / CEP-Zeo / Hygro

    Plasmid

    #16622

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Insert

    TGF beta type II receptor (TGFBR2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. 2XMyc-LRRK2-Kinase

    Plasmid

    #25071

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the kinase domain aa 1863-2218

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-tTA

    Plasmid

    #193338

    Purpose

    AAV-mediated expression of tTA under the CAG promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EC59103

    Plasmid

    #191785

    Purpose

    LbCas12a expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas12a+ 8 introns-T-E9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D156R

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-PTBP1CCtoSS

    Plasmid

    #89157

    Purpose

    Recombinant expression of PTBP1 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-PIK3CA-R88Q

    Plasmid

    #116538

    Purpose

    Lentiviral expression of PIK3CA R88Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R88Q

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge