166,778 results

1. VPR_dCas9

   Plasmid

   #134601

   Purpose

   Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

   Depositor

   Lasse Ebdrup Pedersen

   Article

   Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

   Insert

   VPR-dCas9-2A-ZsGreen-DR
   

   Tags

   VPR and ZsGreen1-DR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Tet3G blasticidin

   Plasmid

   #128061

   Purpose

   Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

   Plasmid

   #107942

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R, and N282A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. psPAX2-D64V

   Plasmid

   #63586

   Purpose

   psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

   Depositor

   David Rawlings, Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Packaging plasmid for integrase deficient lentiviral vectors
   

   Use

   Integrase deficient lentiviral packaging vector

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEf1a-superDn29-dCas9-P2A-GFP

   Plasmid

   #247156

   Purpose

   Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   superDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC67908

   Plasmid

   #211795

   Purpose

   Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   HptII, Cas12a, GR-GRF-GIF
   

   Expression

   Plant

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-hMALAT1

   Plasmid

   #118580

   Purpose

   to overexpress human MALAT1 in mammalian cells

   Depositor

   Jianjun Zhao

   Article

   Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

   Insert

   MALAT1 (MALAT1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC101-AmpR EV

    Plasmid

    #208184

    Purpose

    empty plasmid vector, non-fluorescent

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Type

    Empty backbone

    Use

    Reporter

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS2-GFP

    Plasmid

    #27121

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Tags

    EGFP, HA, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-myr-AKT-HA

    Plasmid

    #31789

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid

    #107738

    Purpose

    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

    Depositor

    Rylan Larsen

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre, dTomato
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CMV-MCS-Puro

    Plasmid

    #219794

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Mutation

    G12V

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) eGFP

    Plasmid

    #129020

    Purpose

    Expresses eGFP

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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