165,214 results

1. pMV306G13+Lux

   Plasmid

   #26160

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   G13 promoter + Bacterial luciferase operon
   

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiV_Cas9_Blast

   Plasmid

   #125592

   Purpose

   Lentiviral expression of spCas9 with blasticidin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

   Plasmid

   #234826

   Purpose

   STITCHR R2Tocc editor

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   nCas9h840a-xten-R2Tocc
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNK3071

   Plasmid

   #219755

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Expression

   Plant

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti6.2_mCherry_mito Grx1-roGFP2

   Plasmid

   #155044

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   mitochondrial targeting sequence and roGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRMC2

   Plasmid

   #68940

   Purpose

   tetracycline-inducible expression vector for Staphylococcus aureus

   Depositor

   Tim Foster

   Article

   Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

   Type

   Empty backbone

   Use

   Tet inducible e. coli/staphylococcus shuttle vect…

   Expression

   Bacterial

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. kiCAP-AAV-BI30

   Plasmid

   #183749

   Purpose

   AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
   

   Use

   AAV

   Mutation

   K449R, and NNSTRGG inserted between 588 and 589 o…

   Promoter

   p41

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-MCS2

    Plasmid

    #46954

    Purpose

    Cloning plasmid for the generation of Knock-In in human cells using rAAV.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV2/9n

    Plasmid

    #112865

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap9
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xHA-TurboID-NLS_pCDNA3

    Plasmid

    #107171

    Purpose

    expresses 3xHA-tagged TurboID in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDY1656 STITCHR Gluc AAVS1 payload

    Plasmid

    #234827

    Purpose

    Gluc exon 2 with 50bp AAVS1 homology arms

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    Gluc exon 2
    

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Promoter

    minimal TATA

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mscv2.2-NLRC4

    Plasmid

    #60199

    Purpose

    Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge