pLenti6/UbC/mSlc7a1
(Plasmid #17224)

• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Backbone

• Vector backbone: pLenti6/UbC/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7600
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: We recommend using Stbl3 E. coli strain (Invitrogen).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Slc7a1
• Species: M. musculus (mouse)
• Insert Size (bp): 1870
• Entrez Gene: Slc7a1 (a.k.a. 4831426K01Rik, AI447493, Atrc-1, Atrc1, Cat1, Rec-1, Rev-1, mCAT-1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: hUBCpro-F (5'-TGAAGCTCCGGTTTTGAACT-3')
• 3′ sequencing primer: pBABE3

Resource Information

• Addgene Notes:
  • NotI digestion
• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry