PurposeRetroviral expression of mouse Klf4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13370||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameKruppel-like factor 4 (gut)
SpeciesM. musculus (mouse)
Insert Size (bp)1425
Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
- Cloning method Unknown
- 5′ sequencing primer pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
- 3′ sequencing primer LNCX-3 (5'-ACCTACAGGTGGGGTCTTTCATTCCC-3') (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Mouse Klf4 includes G372A, A459C, G527C, G633C, G1021C, C1305G and T1368C mutations.
All of substitutions can be found in EST database of NCBI. So we believe that these differences are caused by SNPs.
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-Klf4 was a gift from Shinya Yamanaka (Addgene plasmid # 13370 ; http://n2t.net/addgene:13370 ; RRID:Addgene_13370)
For your References section:Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174