PurposeRetroviral expression of mouse Oct3/4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13366||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePOU domain, class 5, transcription factor 1
SpeciesM. musculus (mouse)
Insert Size (bp)1134
Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Mouse Oct3/4 includes C91T mutation.
We found that EST clones including substitution C91 to T in NCBI database. So we believe that these substitutions are ascribed to SNPs.
CJ069184 RIKEN full-length enriched mouse cDNA library, CRL-2070
CJ069414 RIKEN full-length enriched mouse cDNA library, CRL-2070
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-Oct3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 13366 ; http://n2t.net/addgene:13366 ; RRID:Addgene_13366)
For your References section:Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174