|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13367||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSRY-box containing gene 2
SpeciesM. musculus (mouse)
Insert Size (bp)960
Entrez GeneSox2 (a.k.a. Sox, Sox-2, lc, lcc, ys, ysb)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If
this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-Sox2 was a gift from Shinya Yamanaka (Addgene plasmid # 13367 ; http://n2t.net/addgene:13367 ; RRID:Addgene_13367)
For your References section:Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174