161,186 results

1. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-ESR1-Y537S

   Plasmid

   #116374

   Purpose

   Lentiviral expression of ESR1 Y537S

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y537S

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-mTagBFP2-T2A-iCre-ERT2

   Plasmid

   #128168

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TC1332

   Plasmid

   #164874

   Purpose

   CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJ7

   Plasmid

   #167136

   Purpose

   A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Kanamycin promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-mito-dsRED2

   Plasmid

   #173071

   Purpose

   mitochondria targeted dsRED2 expressed off of CAG promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ1

   Plasmid

   #167130

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Frr promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

   Plasmid

   #52536

   Purpose

   Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

   Depositor

   Scott Sternson

   Article

   Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

   Has Service

   AAV1

   Insert

   hM4D-2a-GFP (CHRM4 Human, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Scarlet I-FRET 10-NeonGreen

   Plasmid

   #139364

   Purpose

   FRET Standard

   Depositor

   Richard Day

   Article

   Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

   Insert

   Scarlet 10AA Neon green
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3HR2

    Plasmid

    #220738

    Purpose

    Component of yeast three hybrid system

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO mouse shRNA 1 rictor

    Plasmid

    #21341

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    rictor shRNA 1 (Rictor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX-Tight_A-CREB_PGK-tdTomato

    Plasmid

    #154264

    Purpose

    Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    dominant negative CREB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight/tetO

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-EWSR1 Stop

    Plasmid

    #84887

    Purpose

    DONOR vector for Gateway cloning of EWSR1

    Depositor

    Aaron Gitler

    Article

    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p CIneo-RL

    Plasmid

    #115366

    Purpose

    Expression vector to produce humanized Renilla luciferase

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBRIT Pax7 FLAG

    Plasmid

    #17521

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-UMI

    Plasmid

    #222694

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 131-2a_LC

    Plasmid

    #215331

    Purpose

    Expression monoclonal antibody 131-2a LC

    Depositor

    Joost Snijder

    Article

    Peng et al bioRxiv 2025.01.04.631317

    Insert

    131-2a_LC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 131-2a_HC

    Plasmid

    #214965

    Purpose

    Expression monoclonal antibody 131-2a

    Depositor

    Joost Snijder

    Article

    Peng et al bioRxiv 2025.01.04.631317

    Insert

    131-2a_HC
    

    Use

    Tags

    his8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Sox2-IRES-Neo

    Plasmid

    #13462

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2_EF1a_TetOn_IRES_mCherry

    Plasmid

    #204155

    Purpose

    Lentivirus backbone expressing rtTA linked to mCherry

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Tet-On 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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