165,214 results

1. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRM98 mKate2 Blast

   Plasmid

   #174720

   Purpose

   Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   mKate2
   BSD
   

   Use

   Lentiviral

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND and MND (off mKate-P2A)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSK9065

   Plasmid

   #84345

   Purpose

   tightly controlled expression of mRFP fusion proteins in S. aureus

   Depositor

   Neville Firth

   Article

   Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

   Type

   Empty backbone

   Tags

   mRFPmars

   Expression

   Bacterial

   Promoter

   Pxyl/tet

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-TSC2

   Plasmid

   #15478

   Depositor

   Do-Hyung Kim

   Article

   Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

   Insert

   TSC2 shRNA (TSC2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-HyPerGRt

   Plasmid

   #173197

   Purpose

   HyPer7 and tdTomato fusion to detect mitochondrial hydrogen peroxide

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

   Insert

   Hyper7-tdTomato
   

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG PTEN

   Plasmid

   #78777

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-UbE2S

   Plasmid

   #15807

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2S (UBE2S Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLM-AMA15.0 Cas9

   Plasmid

   #138944

   Purpose

   Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

   Insert

   p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
   

   Use

   CRISPR and Synthetic Biology; Ama1 autonomously r…

   Tags

   NLS

   Promoter

   p40S (AN0465) Aspergillus nidulans

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mAIM2-Flag

   Plasmid

   #51537

   Purpose

   Express Flag-tagged mAIM2 in mammalian cells

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK562 (pDS-GFP)

    Plasmid

    #183129

    Purpose

    Control plasmid that expresses off-target dsRNA

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    GFPmut3 (no RBS)
    

    Use

    Synthetic Biology

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST17-UbE2L3

    Plasmid

    #15795

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK800

    Plasmid

    #179209

    Purpose

    GFP dropout for assembling a double-stranded RNA expression plasmid

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET-30a-NSP14-SARS-CoV-2

    Plasmid

    #236423

    Purpose

    bacterial protein expression of NSP14

    Depositor

    Michele Pagano

    Article

    Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

    Insert

    NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pW34

    Plasmid

    #158210

    Purpose

    LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    LuxABCDE
    

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HaloTag-Ubiquitin Fusion Vector

    Plasmid

    #236943

    Purpose

    Express HaloTag(R)-Ubiquitin Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    Ubiquitin (UBC Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST17-UbE2C

    Plasmid

    #15779

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2C (UBE2C Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pCI-neo-hCG

    Plasmid

    #16574

    Depositor

    Bert Vogelstein

    Article

    Shih et al Nat Med. 2000 Jun . 6(6):711-4.

    Insert

    Beta-hCG (CGB3 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pBbB19k-GFP

    Plasmid

    #126135

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23105

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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