pCI flag PCAF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8941||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2579
Entrez GeneKAT2B (a.k.a. CAF, P/CAF, PCAF)
/ Fusion Protein
- flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Compared to GenBank reference sequence NP_003875.3, Serine-2 is actually Valine-2 immediately after the FLAG tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI flag PCAF was a gift from Yoshihiro Nakatani (Addgene plasmid # 8941 ; http://n2t.net/addgene:8941 ; RRID:Addgene_8941)
For your References section:A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Yang XJ, Ogryzko VV, Nishikawa J, Howard BH, Nakatani Y. Nature. 1996 Jul 25. 382(6589):319-24. 10.1038/382319a0 PubMed 8684459