161,202 results

1. pLG033 (ietAS)

   Plasmid

   #157911

   Purpose

   Expresses the ietAS defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ietAS (ATPase + serine protease)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

   Plasmid

   #203770

   Purpose

   For production of template RNA for use in PRINT, mCherry under CMV promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for mCherry
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

   Plasmid

   #124707

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD (E488Q)_NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutant

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM5409

   Plasmid

   #132662

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PaadA and PconII

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215015

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1 human cofilin S3E

   Plasmid

   #50861

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. -5.5kb-UCP1-GFP

   Plasmid

   #104585

   Purpose

   Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

   Depositor

   Irfan Lodhi

   Article

   Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

   Insert

   UCP1 promoter (Ucp1 Mouse)
   

   Use

   Tags

   TurboGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UCP1

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BLBP-GFP

   Plasmid

   #63174

   Purpose

   Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

   Depositor

   Bart De Strooper

   Article

   Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

   Insert

   BLBP (Fabp7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   1700bp upstream of BLBP gene

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS puro-GFP-BamHI-IRES-mCherry

   Plasmid

   #232247

   Purpose

   AcGFP1-BamHI-IRES-mCherry empty backbone

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cerulean-2xFv-hRIPK3

    Plasmid

    #106079

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    Cerulean-2xFv-hRIPK3 (RIPK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLD-puro-Cc-PRDX5WT-VA

    Plasmid

    #98688

    Purpose

    Lentiviral expression of human PRDX5-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-GFP11(x7)-Actin

    Plasmid

    #181967

    Purpose

    Human beta actin tagged with 7 copies of GFP11.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11(x7)-Actin (ACTB )
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcBac1.tR4-AcdRS82

    Plasmid

    #174101

    Purpose

    Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    M. barkeri tRNA synthetase for acridone82
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    CMV

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

    Plasmid

    #211466

    Purpose

    Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    mito-mCherry-GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 BoMo N-1F

    Plasmid

    #203761

    Purpose

    For expression of N-flag tagged BoMo R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LiOn-CAG∞IRFP

    Plasmid

    #154017

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    IRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD3sE_SC12

    Plasmid

    #219720

    Purpose

    DV3 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV3 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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