162,717 results

1. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

   Plasmid

   #217711

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA1 (ADGRA1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-6

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK025

   Plasmid

   #236572

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3TP-lux

   Plasmid

   #11767

   Depositor

   Jeff Wrana, Joan Massague

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   3TP
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. gTBEv3

   Plasmid

   #220619

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Rab5A S34N

   Plasmid

   #28045

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab5A (RAB5A Human)
   

   Use

   Tags

   EGFP on N-term

   Expression

   Mammalian

   Mutation

   S34N

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6MQBI163 SGLT2

   Plasmid

   #194147

   Purpose

   Expresses SGLT2 protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC5A2 (SLC5A2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-TetOne-Puro-MCL1

   Plasmid

   #202663

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU2-AmyE-Pveg-eGFP

   Plasmid

   #112792

   Purpose

   Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   Pveg-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2535

   Plasmid

   #142956

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX6 (SOX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-PIK3C2G

    Plasmid

    #171946

    Purpose

    Expresses PIK3C2G in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    PIK3C2G (PIK3C2G Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-J chain

    Plasmid

    #145146

    Purpose

    Mammalian expression plasmid for J chain of polymeric antibody classes

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    immunoglobulin J chain (JCHAIN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FU- dio PSD95-mCherry- W

    Plasmid

    #73919

    Purpose

    Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    PSD-95-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi-C

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3505

    Plasmid

    #144981

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

    Plasmid

    #194171

    Purpose

    Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-Associated Protein Tau 0N4R
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-emiRFP670

    Plasmid

    #197200

    Purpose

    Expresses the protein of emiRFP670 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    emiRFP670
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-TK-5UTR-BsmBI-Luciferase

    Plasmid

    #114670

    Purpose

    For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

    Depositor

    Lynne Postovit

    Article

    Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

    Insert

    LacZ
    

    Use

    Luciferase

    Tags

    for cloning 5' UTR-Luciferase fusions

    Expression

    Mammalian

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HaloTag-A2A-adenosine receptor

    Plasmid

    #66995

    Purpose

    A2A-adenosine receptor tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    

    Use

    Tags

    Human Growth hormone Signal Sequence- HaloTag

    Expression

    Mammalian

    Mutation

    The signal sequence from human growth hormone was…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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