166,778 results

1. pPAP004

   Plasmid

   #160911

   Purpose

   E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   lacZ selection cassette
   

   Expression

   Yeast

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHA2

   Plasmid

   #85710

   Purpose

   This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

   Depositor

   Ulrich Koszinowski, Martin Messerle

   Article

   Adler et al J Virol. 2000 Aug;74(15):6964-74.

   Type

   Empty backbone

   Use

   Cre/Lox; Herpesvirus cloning

   Expression

   Bacterial

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 TAP DHX36

   Plasmid

   #53547

   Purpose

   mammalian expression of DHX36

   Depositor

   Kathleen Collins

   Article

   Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   TAP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CD74-ROS1 nonmutant

   Plasmid

   #183813

   Purpose

   Express CD74-ROS1 fusion (C6;R34) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. #2.4 post-G-SynapShot-mScarlet

   Plasmid

   #214720

   Purpose

   Expresses green #2.4 post-SynapShot and mScarlet

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 post-G-SynapShot
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIB4

   Plasmid

   #25453

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Available Since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-R-iLACCO1

   Plasmid

   #208025

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSFLPe

   Plasmid

   #31130

   Depositor

   Gerhart Ryffel

   Article

   Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

   Insert

   FLP recombinase
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid

    #55613

    Purpose

    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Silent mutations have been incorporated into the …

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a-hPKM2

    Plasmid

    #44242

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-ArchT-BFP2-TSERex

    Plasmid

    #123312

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    ArchT-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-AHPH-DM

    Plasmid

    #71368

    Purpose

    Location sensor for RhoA-GTP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    C terminus of Anillin (ANLN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A740D and E758K

    Promoter

    CMV

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM290

    Plasmid

    #46038

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    S2G introduced during cloning

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUBQ:Citrine-TurboID-3xMyc

    Plasmid

    #199244

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID-3xMyc
    

    Tags

    TurboID-3xMyc

    Expression

    Plant

    Promoter

    Arabidopsis UBQ10 promoter with intron

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-hKCNQ3-P2A-EGFP

    Plasmid

    #209195

    Purpose

    Cre-dependent expression of Kcnq3 and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3414

    Plasmid

    #144890

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROG1 (NEUROG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS-memb-mCherry

    Plasmid

    #53750

    Purpose

    Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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