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(Plasmid #49435)


Item Catalog # Description Quantity Price (USD)
Plasmid 49435 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    VQ Ad5CMV K-NpA
  • Backbone manufacturer
    ViraQuest Inc.
  • Backbone size w/o insert (bp) 5217
  • Total vector size (bp) 5970
  • Modifications to backbone
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
  • Insert Size (bp)
  • Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's GFP
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'GGCGTGTACGGTGGGAGGTCTATA-3'
  • 3′ sequencing primer 5'-TTAAAAAACCTCCCCACCTCCCCC-3'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We inserted mutations into Clontech's eGFP gene to convert it into roGFP, and then ligated it into a ViraQuest plasmid to permit expression in mammalian cells under the CMV promoter, and also to permit generation of adenovirus by ViraQuest in Iowa.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cyto-roGFP was a gift from Paul Schumacker (Addgene plasmid # 49435 ; ; RRID:Addgene_49435)
  • For your References section:

    Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, Schumacker PT. Circ Res. 2010 Feb 19;106(3):526-35. doi: 10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17. 10.1161/CIRCRESAHA.109.206334 PubMed 20019331