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GPD-roGFP
(Plasmid #49436)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49436 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    VQ Ad5CMV K-NpA
  • Backbone manufacturer
    ViraQuest Inc.
  • Backbone size w/o insert (bp) 5217
  • Total vector size (bp) 6200
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPD-roGFP2
  • Alt name
    IMS-roGFP
  • Species
    Synthetic
  • Insert Size (bp)
    3150
  • Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's GFP
  • Promoter CMV
  • Tag / Fusion Protein
    • Glycerol phosphate dehydrogenase, 5' to roGFP2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer 5'GGCGTGTACGGTGGGAGGTCTATA-3'
  • 3′ sequencing primer 5'-TTAAAAAACCTCCCCACCTCCCCC-3'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We inserted mutations into Clontech's eGFP gene to convert it into roGFP, and cut the roGFP from the Clontech backbone. We ligated cDNA encoding glycerol phosphate dehydrogenase (GPD)(5') to the roGFP dDNA (3') - a ClaI site separates the GPD and roGFP2 genes. The GPD-roGFP construct was ligated into the VQ vector between XhoI (5') and BamHI (3') sites.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GPD-roGFP was a gift from Paul Schumacker (Addgene plasmid # 49436 ; http://n2t.net/addgene:49436 ; RRID:Addgene_49436)
  • For your References section:

    Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, Schumacker PT. Circ Res. 2010 Feb 19;106(3):526-35. doi: 10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17. 10.1161/CIRCRESAHA.109.206334 PubMed 20019331