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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_CoV2_D18

Plasmid

#170442

Purpose

Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)

Use

Tags

NA

Expression

Mammalian

Mutation

18aa deletion in c-terminal tail

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Tsg101

Plasmid

#21505

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

Tsg101 (TSG101 Human)

Use

Tags

Cherry*

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Lambda promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Myr_mCherry-2A-eGFP

Plasmid

#194877

Purpose

Ratiometric Myr/Palm_mCherry control probe

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

Myristoylated/Palmitoylated_mCherry-T2A-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFlpStop-attB-UAS-2.1-tdTom

Plasmid

#88910

Purpose

attB FlpStop plasmid, for insertion into MiMIC site

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Insert

FlpStop

Use

Tags

Expression

Insect

Mutation

Promoter

5XUAS

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-NES_pCDNA3

Plasmid

#107169

Purpose

expresses V5-tagged TurboID in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-gp32-H6

Plasmid

#163912

Purpose

Expresses T4 gp32 for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

gp32

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCrl

Plasmid

#122443

Purpose

Expresses entire mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Article

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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