166,778 results

1. TFORF0070

   Plasmid

   #141505

   Purpose

   Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CSDE1 (CSDE1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

   Plasmid

   #223087

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-GreenPy1Highest

   Plasmid

   #239049

   Purpose

   Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1Highest
   

   Expression

   Bacterial

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-GreenPy1High

   Plasmid

   #239050

   Purpose

   Bacterial expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1High
   

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PDX1-2A-eGFP

   Plasmid

   #66964

   Purpose

   PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   pancreatic and duodenal homeobox 1 (PDX1 Human)
   

   Use

   Unspecified

   Tags

   2A-eGFP

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-HypaCas9

   Plasmid

   #108302

   Purpose

   pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-iLACCO1

   Plasmid

   #218821

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1
   

   Expression

   Mammalian

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLN-AIDA-Tyr1

   Plasmid

   #192831

   Purpose

   IPTG inducible AIDA-Tyrosinase expression

   Depositor

   André Studart

   Article

   Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

   Insert

   AIDA-Tyr1
   

   Expression

   Bacterial

   Promoter

   pL-lac

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid

    #100597

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pFOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGHYB

    Plasmid

    #87447

    Purpose

    Pichia pastoris expression plasmid

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-P05

    Plasmid

    #120016

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-zSpRY

    Plasmid

    #179317

    Purpose

    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Luciferase

    Plasmid

    #213979

    Purpose

    Expresses Doxy Inducible Luciferase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    6xHis affinity tag

    Expression

    Bacterial and Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-Nck1

    Plasmid

    #45903

    Depositor

    Louise Larose

    Article

    Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

    Insert

    Nck-1 (NCK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBAD-eLACCO1.1

    Plasmid

    #167944

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1095

    Plasmid

    #143923

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EN1 (EN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TRAIL-R2

    Plasmid

    #61383

    Purpose

    Expresses TRAIL receptor 2

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 2 (TNFRSF10B Human)
    

    Tags

    none

    Expression

    Mammalian

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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