166,778 results

1. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC5941-PacI

   Plasmid

   #44182

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLib6.4B-Aalb_743

   Plasmid

   #176676

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti.pFOXA2.5'.GFP

   Plasmid

   #59407

   Purpose

   expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

   Depositor

   Kai-Christian Sonntag

   Article

   Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

   Insert

   approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   FOXA2

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ptc1-YFP

   Plasmid

   #58456

   Purpose

   expression of mouse Ptc1-YFP

   Depositor

   Matthew Scott

   Article

   Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

   Insert

   Ptc1 (Ptch1 Mouse)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCMV eGFP hPGK BSD

   Plasmid

   #188519

   Purpose

   Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF_dCas9-VP64

   Plasmid

   #68417

   Purpose

   Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and VP64

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-T-GECO1

   Plasmid

   #191616

   Purpose

   Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

   Depositor

   Robert Campbell

   Article

   Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

   Insert

   T-GECO1
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-iLACCO1

    Plasmid

    #218817

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Expression

    Bacterial

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-iLACCO1.1

    Plasmid

    #218818

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.1
    

    Expression

    Bacterial

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-iLACCO1.2

    Plasmid

    #218819

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Expression

    Bacterial

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-DiLACCO1

    Plasmid

    #218820

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate control biosensor

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    DiLACCO1
    

    Expression

    Bacterial

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKHR4

    Plasmid

    #74592

    Purpose

    an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Type

    Empty backbone

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-XPR1_STOP

    Plasmid

    #161472

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    XPR1 (XPR1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCDNA5_miniTurbo-C12orf49

    Plasmid

    #155111

    Purpose

    transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAi14-GFPNLS-MC

    Plasmid

    #87114

    Purpose

    minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFPNLSPpA
    

    Use

    CRISPR

    Promoter

    nEF

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-STX17ΔNTD

    Plasmid

    #89941

    Purpose

    Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

    Insert

    human syntaxin17 lacking N-terminal domain (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    deleted amino acids 1-161

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RGG-BcLOV-mCh

    Plasmid

    #208276

    Purpose

    Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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