Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,069 results

Showing: 781 - 800 of 177069 results

  4. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Publication
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    20859-plasmid-map-sequence-id-220527

    Enlarge

  5. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    112202-plasmid-map-sequence-id-334619

    Enlarge

  6. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid
    #83841
    Purpose
    Fluorescent probe for G1/S transition and M/G1 transition, one of two plasmids for the FUCCI4 cell cycle reporter
    Depositor
    Michael Lin
    Publication
    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Insert
    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    83841-plasmid-map-sequence-id-518123

    Enlarge

  18. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Publication
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    171782-plasmid-map-sequence-id-443047

    Enlarge

Showing: 781 - 800 of 177069 results