161,202 results

1. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET51b(+)_SNAP-tag2

   Plasmid

   #226512

   Purpose

   Bacterial cell expression of SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   SNAP-tag2
   

   Use

   Tags

   His-tag (10x) and Strep-Tag II,

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAV1K-T5-gfp

   Plasmid

   #26702

   Depositor

   Ichiro Matsumura

   Article

   Bryksin et al PLoS One. 2010 . 5(10):e13244.

   Insert

   T5lacOlacOeGFP biobrick
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-mCherry-CAAX

   Plasmid

   #129285

   Purpose

   Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   Fluorescent reporter mCherry fused to CAAX polybasic sequence
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTagBFP2-pBAD

   Plasmid

   #54572

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, T7, and mTagBFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. V5-TurboID-NES_pCDNA3

   Plasmid

   #107169

   Purpose

   expresses V5-tagged TurboID in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PS6K-mcherry-iATPsnFR1.1

    Plasmid

    #227238

    Purpose

    Inducible ATP sensor for E. coli

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-Myc3-Nrf2

    Plasmid

    #21555

    Purpose

    Mammalian expression of human Nrf2 with Myc tag

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dTomato-2xrGBD

    Plasmid

    #129625

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    dTomato-2xrGBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NFAT binding site

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Use

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tol2-CAG::Palmbow

    Plasmid

    #158993

    Purpose

    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Palmbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B; oth…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge