R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters.
Chen L, Chen JY, Zhang X, Gu Y, Xiao R, Shao C, Tang P, Qian H, Luo D, Li H, Zhou Y, Zhang DE, Fu XD
Mol Cell. 2017 Nov 16;68(4):745-757.e5. doi: 10.1016/j.molcel.2017.10.008. Epub 2017 Nov 2. PubMed Article
Chen L, Chen JY, Zhang X, Gu Y, Xiao R, Shao C, Tang P, Qian H, Luo D, Li H, Zhou Y, Zhang DE, Fu XD
Mol Cell. 2017 Nov 16;68(4):745-757.e5. doi: 10.1016/j.molcel.2017.10.008. Epub 2017 Nov 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111904 | ppyCAG_RNaseH1_D210N | Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1 |
| 111905 | ppyCAG_RNaseH1_WKKD | Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids |
| 111906 | ppyCAG_RNaseH1_WT | Express RNASEH1 in mammalian cell. |