Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

AAVS1_Puro_PGK1_3xFLAG_Twin_Strep Citations (20)

Originally described in: A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
PubMed Journal

Articles Citing AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Articles
Preparation and Analysis of Native Chromatin-Modifying Complexes. Doyon Y, Cote J. Methods Enzymol. 2016;573:303-18. doi: 10.1016/bs.mie.2016.01.017. Epub 2016 Mar 16. PubMed
Marker-free coselection for CRISPR-driven genome editing in human cells. Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. PubMed

Associated Plasmids

Murine knockin model for progranulin-deficient frontotemporal dementia with nonsense-mediated mRNA decay. Nguyen AD, Nguyen TA, Zhang J, Devireddy S, Zhou P, Karydas AM, Xu X, Miller BL, Rigo F, Ferguson SM, Huang EJ, Walther TC, Farese RV Jr. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2849-E2858. doi: 10.1073/pnas.1722344115. Epub 2018 Mar 6. PubMed
Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing bisphosphonates. Yu Z, Surface LE, Park CY, Horlbeck MA, Wyant GA, Abu-Remaileh M, Peterson TR, Sabatini DM, Weissman JS, O'Shea EK. Elife. 2018 May 10;7. pii: 36620. doi: 10.7554/eLife.36620. PubMed
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PubMed

Associated Plasmids

Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures. Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, Skrzypczak M, Aymard F, Fongang B, Dojer N, Iacovoni JS, Rowicka M, Ginalski K, Cote J, Legube G. Mol Cell. 2018 Oct 18;72(2):250-262.e6. doi: 10.1016/j.molcel.2018.08.020. Epub 2018 Sep 27. PubMed
Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway. Nogimori T, Nishiura K, Kawashima S, Nagai T, Oishi Y, Hosoda N, Imataka H, Kitamura Y, Kitade Y, Hoshino SI. Nucleic Acids Res. 2019 Jan 10;47(1):432-449. doi: 10.1093/nar/gky1087. PubMed
Measurement and Analysis of Lysine Acetylation by KAT Complexes In Vitro and In Vivo. Lashgari A, Lambert JP, Cote J. Methods Mol Biol. 2019;1983:57-77. doi: 10.1007/978-1-4939-9434-2_5. PubMed
Aberrant expression of select piRNA-pathway genes does not reactivate piRNA silencing in cancer cells. Genzor P, Cordts SC, Bokil NV, Haase AD. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11111-11112. doi: 10.1073/pnas.1904498116. Epub 2019 May 20. PubMed
Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ. Cell. 2019 Sep 19;179(1):251-267.e24. doi: 10.1016/j.cell.2019.08.013. PubMed

Associated Plasmids

Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6. Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A. Mol Cell. 2019 Dec 19;76(6):922-937.e7. doi: 10.1016/j.molcel.2019.09.012. Epub 2019 Oct 8. PubMed
LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7. PubMed
Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression. Mejhert N, Kuruvilla L, Gabriel KR, Elliott SD, Guie MA, Wang H, Lai ZW, Lane EA, Christiano R, Danial NN, Farese RV Jr, Walther TC. Mol Cell. 2020 Mar 19;77(6):1251-1264.e9. doi: 10.1016/j.molcel.2020.01.014. Epub 2020 Feb 4. PubMed
Approaches to Study Native Chromatin-Modifying Complex Activities and Functions. Galloy M, Lachance C, Cheng X, Distefano-Gagne F, Cote J, Fradet-Turcotte A. Front Cell Dev Biol. 2021 Sep 16;9:729338. doi: 10.3389/fcell.2021.729338. eCollection 2021. PubMed
Development of a versatile HPLC-based method to evaluate the activation status of small GTPases. Araki M, Yoshimoto K, Ohta M, Katada T, Kontani K. J Biol Chem. 2021 Nov 18:101428. doi: 10.1016/j.jbc.2021.101428. PubMed

Associated Plasmids

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation. Poetz F, Corbo J, Levdansky Y, Spiegelhalter A, Lindner D, Magg V, Lebedeva S, Schweiggert J, Schott J, Valkov E, Stoecklin G. Nat Commun. 2021 Dec 9;12(1):7175. doi: 10.1038/s41467-021-27471-6. PubMed
Evaluation of in vitro fibroblast migration by electrospun triple-layered PU-CA/gelatin.PRGF/PU-CA scaffold using an AAVS1 targeted EGFP reporter cell line. Shams F, Moravvej H, Hosseinzadeh S, Kazemi B, Rajabibazl M, Rahimpour A. Bioimpacts. 2022;12(3):219-231. doi: 10.34172/bi.2021.43. Epub 2021 Aug 30. PubMed
Identification of HCN1 as a 14-3-3 client. Lankford C, Houtman J, Baker SA. PLoS One. 2022 Jun 9;17(6):e0268335. doi: 10.1371/journal.pone.0268335. eCollection 2022. PubMed
A nascent peptide code for translational control of mRNA stability in human cells. Burke PC, Park H, Subramaniam AR. Nat Commun. 2022 Nov 11;13(1):6829. doi: 10.1038/s41467-022-34664-0. PubMed
O-GlcNAcylation regulates neurofilament-light assembly and function and is perturbed by Charcot-Marie-Tooth disease mutations. Huynh DT, Hu J, Schneider JR, Tsolova KN, Soderblom EJ, Watson AJ, Chi JT, Evans CS, Boyce M. bioRxiv. 2023 Feb 22:2023.02.22.529563. doi: 10.1101/2023.02.22.529563. Preprint. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.