A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y
Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68375 | AAVS1_Puro_PGK1_3xFLAG_Twin_Strep | Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging. |
| 79877 | eSpCas9(1.1)_No_FLAG | FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid. |
| 79888 | eSpCas9(1.1)_No_FLAG_AAVS1_T2 | Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus. |
| 79891 | FancF_N_FS_v2_Donor | N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep |
| 79892 | SP_gRNA_pUC19_N_FancF_Left | Human gRNA expression vector targeting FANCF (site 1 left) |
| 79899 | EZH2-TALEN_Left_No_FLAG | Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2 |
| 79900 | EZH2-TALEN_Right_No_FLAG | Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2 |
| 79901 | EZH2_N_FS_v2_Donor | N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep |
| 79902 | AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2 | Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus |
| 92099 | AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep | Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System. |