Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y
Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68375AAVS1_Puro_PGK1_3xFLAG_Twin_StrepDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
Loading...
79877eSpCas9(1.1)_No_FLAGFLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.
Loading...
79888eSpCas9(1.1)_No_FLAG_AAVS1_T2Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.
Loading...
79891FancF_N_FS_v2_DonorN-terminal tagging of human FANCF with 3xFLAG_Twin_Strep
Loading...
79892SP_gRNA_pUC19_N_FancF_LeftHuman gRNA expression vector targeting FANCF (site 1 left)
Loading...
79899EZH2-TALEN_Left_No_FLAGUntagged EZH2-TALEN_Left to target the N-terminus of human EZH2
Loading...
79900EZH2-TALEN_Right_No_FLAGUntagged EZH2-TALEN_Right to target the N-terminus of human EZH2
Loading...
79901EZH2_N_FS_v2_DonorN-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep
Loading...
79902AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus
Loading...
92099AAVS1_Puro_Tet3G_3xFLAG_Twin_StrepDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
Loading...