Purpose(Empty Backbone) Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79888||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
- Promoter CBh
/ Fusion Protein
- Untagged eSpCas9(1.1)
Growth in Bacteria
Copy numberHigh Copy
This plasmid is derived from eSpCas9(1.1) (Plasmid #71814) from Feng Zhang's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications.
The gRNA sequence is derived from gRNA_AAVS1-T2 (Plasmid #41818) from George Church's lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:eSpCas9(1.1)_No_FLAG_AAVS1_T2 was a gift from Yannick Doyon (Addgene plasmid # 79888 ; http://n2t.net/addgene:79888 ; RRID:Addgene_79888)
For your References section:A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817