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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
(Plasmid #68375)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 68375-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size (bp) 2364
  • Modifications to backbone
    Insert was cloned into PvuII digested pUC19 (loss of 322bp including MCS)
  • Vector type
    Mammalian Expression, CRISPR, TALEN ; ZFN
  • Promoter human PGK1
  • Selectable markers
    Puromycin
  • Tags / Fusion Proteins
    • 3xFLAG_Twin_Strep (N terminal on backbone)
    • 3xFLAG_Twin_Strep (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A detailed protocol for purification of protein complexes tagged with this vector can be found here: https://www.ncbi.nlm.nih.gov/pubmed/27372759

Information for Cloning Grade DNA (Catalog # 68375-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep was a gift from Yannick Doyon (Addgene plasmid # 68375 ; http://n2t.net/addgene:68375 ; RRID:Addgene_68375)
  • For your References section:

    A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817