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AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
(Plasmid #92099)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92099 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size (bp) 2364
  • Modifications to backbone
    Insert was cloned into PvuII digested pUC19 (loss of 322bp including MCS)
  • Vector type
    Mammalian Expression, CRISPR, TALEN ; ZFN
  • Promoter Tet-On 3G Bidirectional
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modified from AAVS1 SA-2A-puro-pA donor (Plasmid #22075). New backbone, overall structure is similar but several modifications have been made through gene synthesis.
  • Terms and Licenses

Depositor Comments

A detailed protocol for purification of protein complexes tagged with this vector can be found here: https://www.ncbi.nlm.nih.gov/pubmed/27372759

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep was a gift from Yannick Doyon (Addgene plasmid # 92099 ; http://n2t.net/addgene:92099 ; RRID:Addgene_92099)
  • For your References section:

    A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817