Purpose(Empty Backbone) Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92099||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2364
Modifications to backboneInsert was cloned into PvuII digested pUC19 (loss of 322bp including MCS)
Vector typeMammalian Expression, CRISPR, TALEN ; ZFN
- Promoter Tet-On 3G Bidirectional
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer See protocol
- 3′ sequencing primer See protocol (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byModified from AAVS1 SA-2A-puro-pA donor (Plasmid #22075). New backbone, overall structure is similar but several modifications have been made through gene synthesis.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A detailed protocol for purification of protein complexes tagged with this vector can be found here: https://www.ncbi.nlm.nih.gov/pubmed/27372759
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep was a gift from Yannick Doyon (Addgene plasmid # 92099 ; http://n2t.net/addgene:92099 ; RRID:Addgene_92099)
For your References section:A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817