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Purposeepisomal expression of FlpO and Cre recombinases
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepOG44
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Backbone manufacturerThermoFisher
- Total vector size (bp) 7971
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Modifications to backboneCMV promoter converted to CAG promoter
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Vector typeMammalian Expression, Cre/Lox, Unspecified ; Episomal expression vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlpO-2A-Cre
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SpeciesS. cerevisiae (budding yeast); P1 bacteriophage
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Insert Size (bp)2364
- Promoter CMV/Chick β-actin (CAG)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCag FlpO-2A-Cre EV was a gift from Joshua Breunig (Addgene plasmid # 129419 ; http://n2t.net/addgene:129419 ; RRID:Addgene_129419) -
For your References section:
Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ. Cell. 2019 Sep 19;179(1):251-267.e24. doi: 10.1016/j.cell.2019.08.013. 10.1016/j.cell.2019.08.013 PubMed 31539496
Map uploaded by the depositor.
