162,717 results

1. L1_HyR-Ck1

   Plasmid

   #136125

   Purpose

   L1 in position 1 (next to LB in L2 construct), for HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:hptII_3t35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. mCherry-iLIDx6

   Plasmid

   #103779

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   mCherry-iLIDx6
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCk4_spacer

   Plasmid

   #136074

   Purpose

   Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pVITRO1-mCardinal-NES

   Plasmid

   #184453

   Purpose

   Cytosolic mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1_lacZgRNA-Ck3

   Plasmid

   #136136

   Purpose

   L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGWB103

   Plasmid

   #68557

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1_Cas9-Ck4

   Plasmid

   #136135

   Purpose

   L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:Cas9_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF0372

   Plasmid

   #141593

   Purpose

   Lentiviral vector for overexpressing the NFYB transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFYB (NFYB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SKIV2L

   Plasmid

   #156014

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SKIV2L (SKIC2 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   D3E

   Promoter

   EF-1a

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNC-Cam3304-RNAi

    Plasmid

    #193401

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_RAF1_WT

    Plasmid

    #82317

    Purpose

    Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHiUGE GS-gRNA (no Cre)

    Plasmid

    #200370

    Purpose

    Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-SFFV-FLAG-TurboID-SQSTM1

    Plasmid

    #223714

    Purpose

    Expresses Flag-tagged TurboID SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLgw EcoDam-V5-RFC1

    Plasmid

    #59209

    Purpose

    Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Lentiviral; Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FERM domain of Ezrin

    Plasmid

    #62380

    Purpose

    Expresses the FERM domain of Ezrin (N terminal portion of the protein)

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Ezrin aa1-309 (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains FERM domain of Ezrin (N terminal portion…

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRVL-3

    Plasmid

    #104581

    Purpose

    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L234A/L235E/G237A and E318A/K320A/K322A

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHiUGE GS-gRNA (hSyn-Cre) mTubb3

    Plasmid

    #200369

    Purpose

    Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Mouse Tubb3 gRNA and Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge