166,778 results

1. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. psPAX2-D64V

   Plasmid

   #63586

   Purpose

   psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

   Depositor

   David Rawlings, Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Packaging plasmid for integrase deficient lentiviral vectors
   

   Use

   Integrase deficient lentiviral packaging vector

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-VN173

   Plasmid

   #22010

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VN173
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Venus residus 1-172

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEf1a-hifiDn29-dCas9-P2A-GFP

   Plasmid

   #247158

   Purpose

   Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-307_LgBiT

    Plasmid

    #191211

    Purpose

    Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    LgBiT (large bit)
    

    Use

    Lentiviral and Luciferase

    Mutation

    AB030246.1 (mutated in PMID 26569370)

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-LSSmGFP

    Plasmid

    #186543

    Purpose

    LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PC1575

    Plasmid

    #232088

    Purpose

    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

    Depositor

    Pranam Chatterjee

    Article

    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

    Insert

    uAb with cloning Esp3I restriction site
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2/8

    Plasmid

    #112864

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap8
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-iCas9-neo

    Plasmid

    #85400

    Purpose

    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Insert

    Flag-iCas9-P2A-GFP
    

    Use

    Lentiviral

    Tags

    Flag-iCas9-P2A-GFP

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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