165,191 results

1. pTRE-Tight-MitoTimer

   Plasmid

   #50547

   Purpose

   Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

   Depositor

   Roberta Gottlieb

   Article

   Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

   Insert

   MitoTimer
   

   Expression

   Mammalian

   Mutation

   F12S mutation in Timer

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-VSVG

   Plasmid

   #64084

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV glycoprotein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-PGK-Cre

   Plasmid

   #172433

   Purpose

   Lentivirus, expresses Cre recombinase

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   Cre
   

   Use

   Lentiviral

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Neo DEST (w531-1)

   Plasmid

   #19067

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIM33-GFP

   Plasmid

   #65399

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   TRIM33 (TRIM33 Human)
   

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK1254

   Plasmid

   #133057

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-FLAG-mCherry-C1-centrin-1

   Plasmid

   #73333

   Purpose

   Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. psfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164477

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

   Plasmid

   #193356

   Purpose

   VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_VP35_EBOV

    Plasmid

    #103050

    Purpose

    Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX313-TP53-WT

    Plasmid

    #118014

    Purpose

    Constitutive expression of wild-type human Tumor Protein p53 (TP53)

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP VAMP7 (1-220)

    Plasmid

    #42316

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 (1-220) (VAMP7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid

    #220800

    Purpose

    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

    Depositor

    Elizabeth Kirby

    Article

    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

    Insert

    Mars1 (Mars1 Mouse)
    

    Use

    Lentiviral

    Tags

    2xFLAG and T2A-mCherry

    Mutation

    mutation in MetRS to change aa 274 from L to G

    Promoter

    EF1a

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSII-EF/mt-(n1)StayGold

    Plasmid

    #185823

    Purpose

    A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    mt-(n1)StayGold
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRL-SV40P

    Plasmid

    #27163

    Purpose

    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

    Depositor

    Ron Prywes

    Article

    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

    Insert

    SV40 promoter
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NRF2

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pComb3XSS

    Plasmid

    #63890

    Purpose

    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    6x His, HA tag, and geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRG (Lenti_sgRNA_EFS_GFP)

    Plasmid

    #65656

    Purpose

    Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter-driven sgRNA expression and EFS promo…

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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