Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


AAV-FLEX-rev-ChR2-tdtomato Citations (42)

Originally described in: A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping.
Atasoy D, Aponte Y, Su HH, Sternson SM J Neurosci. 2008 Jul 9. 28(28):7025-30.
PubMed Journal

Articles Citing AAV-FLEX-rev-ChR2-tdtomato

Visual function in mice with photoreceptor degeneration and transgenic expression of channelrhodopsin 2 in ganglion cells. Thyagarajan S, van Wyk M, Lehmann K, Lowel S, Feng G, Wassle H. J Neurosci. 2010 Jun 30;30(26):8745-58. doi: 10.1523/JNEUROSCI.4417-09.2010. PubMed
AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training. Aponte Y, Atasoy D, Sternson SM. Nat Neurosci. 2011 Mar;14(3):351-5. doi: 10.1038/nn.2739. Epub 2010 Jan 5. PubMed
Hunger states switch a flip-flop memory circuit via a synaptic AMPK-dependent positive feedback loop. Yang Y, Atasoy D, Su HH, Sternson SM. Cell. 2011 Sep 16;146(6):992-1003. doi: 10.1016/j.cell.2011.07.039. PubMed
Gain control by layer six in cortical circuits of vision. Olsen SR, Bortone DS, Adesnik H, Scanziani M. Nature. 2012 Feb 22;483(7387):47-52. doi: 10.1038/nature10835. PubMed
Optogenetic mimicry of the transient activation of dopamine neurons by natural reward is sufficient for operant reinforcement. Kim KM, Baratta MV, Yang A, Lee D, Boyden ES, Fiorillo CD. PLoS One. 2012;7(4):e33612. doi: 10.1371/journal.pone.0033612. Epub 2012 Apr 10. PubMed
Optogenetic probing of fast glutamatergic transmission from hypocretin/orexin to histamine neurons in situ. Schone C, Cao ZF, Apergis-Schoute J, Adamantidis A, Sakurai T, Burdakov D. J Neurosci. 2012 Sep 5;32(36):12437-43. doi: 10.1523/JNEUROSCI.0706-12.2012. PubMed
Target-specific suppression of GABA release from parvalbumin interneurons in the basolateral amygdala by dopamine. Chu HY, Ito W, Li J, Morozov A. J Neurosci. 2012 Oct 17;32(42):14815-20. doi: 10.1523/JNEUROSCI.2997-12.2012. PubMed
Rapid, activity-independent turnover of vesicular transmitter content at a mixed glycine/GABA synapse. Apostolides PF, Trussell LO. J Neurosci. 2013 Mar 13;33(11):4768-81. doi: 10.1523/JNEUROSCI.5555-12.2013. PubMed
Inhibition of inhibition in visual cortex: the logic of connections between molecularly distinct interneurons. Pfeffer CK, Xue M, He M, Huang ZJ, Scanziani M. Nat Neurosci. 2013 Aug;16(8):1068-76. doi: 10.1038/nn.3446. Epub 2013 Jun 30. PubMed
Tuned thalamic excitation is amplified by visual cortical circuits. Lien AD, Scanziani M. Nat Neurosci. 2013 Sep;16(9):1315-23. doi: 10.1038/nn.3488. Epub 2013 Aug 11. PubMed
Equalizing excitation-inhibition ratios across visual cortical neurons. Xue M, Atallah BV, Scanziani M. Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. Epub 2014 Jun 22. PubMed

Associated Plasmids

drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus. Puighermanal E, Biever A, Espallergues J, Gangarossa G, De Bundel D, Valjent E. Hippocampus. 2015 Jul;25(7):858-75. doi: 10.1002/hipo.22408. Epub 2015 Feb 11. PubMed
Callosal projections drive neuronal-specific responses in the mouse auditory cortex. Rock C, Apicella AJ. J Neurosci. 2015 Apr 29;35(17):6703-13. doi: 10.1523/JNEUROSCI.5049-14.2015. PubMed
Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone. Hooper A, Maguire J. Hippocampus. 2015 Jul 1. doi: 10.1002/hipo.22487. PubMed
Increasing the effectiveness of intracerebral injections in adult and neonatal mice: a neurosurgical point of view. Mathon B, Nassar M, Simonnet J, Le Duigou C, Clemenceau S, Miles R, Fricker D. Neurosci Bull. 2015 Dec;31(6):685-96. doi: 10.1007/s12264-015-1558-0. Epub 2015 Sep 7. PubMed
Bidirectional Regulation of Innate and Learned Behaviors That Rely on Frequency Discrimination by Cortical Inhibitory Neurons. Aizenberg M, Mwilambwe-Tshilobo L, Briguglio JJ, Natan RG, Geffen MN. PLoS Biol. 2015 Dec 2;13(12):e1002308. doi: 10.1371/journal.pbio.1002308. eCollection 2015 Dec. PubMed
Optogenetic Control of Mouse Outer Hair Cells. Wu T, Ramamoorthy S, Wilson T, Chen F, Porsov E, Subhash H, Foster S, Zhang Y, Omelchenko I, Bateschell M, Wang L, Brigande JV, Jiang ZG, Mao T, Nuttall AL. Biophys J. 2016 Jan 19;110(2):493-502. doi: 10.1016/j.bpj.2015.11.3521. PubMed
A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery. Hung ME, Leonard JN. J Extracell Vesicles. 2016 May 13;5:31027. doi: 10.3402/jev.v5.31027. eCollection 2016. PubMed

Associated Plasmids

High-fidelity optical excitation of cortico-cortical projections at physiological frequencies. Hass CA, Glickfeld LL. J Neurophysiol. 2016 Nov 1;116(5):2056-2066. doi: 10.1152/jn.00456.2016. Epub 2016 Aug 3. PubMed
Central Control Circuit for Context-Dependent Micturition. Hou XH, Hyun M, Taranda J, Huang KW, Todd E, Feng D, Atwater E, Croney D, Zeidel ML, Osten P, Sabatini BL. Cell. 2016 Sep 22;167(1):73-86.e12. doi: 10.1016/j.cell.2016.08.073. PubMed

Associated Plasmids

Cortico-fugal output from visual cortex promotes plasticity of innate motor behaviour. Liu BH, Huberman AD, Scanziani M. Nature. 2016 Oct 20;538(7625):383-387. doi: 10.1038/nature19818. Epub 2016 Oct 12. PubMed
Decrease of SYNGAP1 in GABAergic cells impairs inhibitory synapse connectivity, synaptic inhibition and cognitive function. Berryer MH, Chattopadhyaya B, Xing P, Riebe I, Bosoi C, Sanon N, Antoine-Bertrand J, Levesque M, Avoli M, Hamdan FF, Carmant L, Lamarche-Vane N, Lacaille JC, Michaud JL, Di Cristo G. Nat Commun. 2016 Nov 9;7:13340. doi: 10.1038/ncomms13340. PubMed
Using a novel PV-Cre rat model to characterize pallidonigral cells and their terminations. Oh YM, Karube F, Takahashi S, Kobayashi K, Takada M, Uchigashima M, Watanabe M, Nishizawa K, Kobayashi K, Fujiyama F. Brain Struct Funct. 2016 Dec 19. PubMed
Cholinergic Grb2-Associated-Binding Protein 1 Regulates Cognitive Function. Lu NN, Tan C, Sun NH, Shao LX, Liu XX, Gao YP, Tao RR, Jiang Q, Wang CK, Huang JY, Zhao K, Wang GF, Liu ZR, Fukunaga K, Lu YM, Han F. Cereb Cortex. 2018 Jul 1;28(7):2391-2404. doi: 10.1093/cercor/bhx141. PubMed
Cell-type-specific role for nucleus accumbens neuroligin-2 in depression and stress susceptibility. Heshmati M, Aleyasin H, Menard C, Christoffel DJ, Flanigan ME, Pfau ML, Hodes GE, Lepack AE, Bicks LK, Takahashi A, Chandra R, Turecki G, Lobo MK, Maze I, Golden SA, Russo SJ. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):1111-1116. doi: 10.1073/pnas.1719014115. Epub 2018 Jan 16. PubMed
Cortical Neural Activity Predicts Sensory Acuity Under Optogenetic Manipulation. Briguglio JJ, Aizenberg M, Balasubramanian V, Geffen MN. J Neurosci. 2018 Feb 21;38(8):2094-2105. doi: 10.1523/JNEUROSCI.2457-17.2017. Epub 2018 Jan 24. PubMed
Sensory experience inversely regulates feedforward and feedback excitation-inhibition ratio in rodent visual cortex. Miska NJ, Richter LM, Cary BA, Gjorgjieva J, Turrigiano GG. Elife. 2018 Oct 12;7. pii: 38846. doi: 10.7554/eLife.38846. PubMed
State-Dependent Subnetworks of Parvalbumin-Expressing Interneurons in Neocortex. Garcia-Junco-Clemente P, Tring E, Ringach DL, Trachtenberg JT. Cell Rep. 2019 Feb 26;26(9):2282-2288.e3. doi: 10.1016/j.celrep.2019.02.005. PubMed
Projection from the Amygdala to the Thalamic Reticular Nucleus Amplifies Cortical Sound Responses. Aizenberg M, Rolon-Martinez S, Pham T, Rao W, Haas JS, Geffen MN. Cell Rep. 2019 Jul 16;28(3):605-615.e4. doi: 10.1016/j.celrep.2019.06.050. PubMed
Rorbeta regulates selective axon-target innervation in the mammalian midbrain. Byun H, Lee HL, Liu H, Forrest D, Rudenko A, Kim IJ. Development. 2019 Jul 22;146(14). pii: 146/14/dev171926. doi: 10.1242/dev.171926. PubMed
Dendritic inhibition differentially regulates excitability of dentate gyrus parvalbumin-expressing interneurons and granule cells. Elgueta C, Bartos M. Nat Commun. 2019 Dec 5;10(1):5561. doi: 10.1038/s41467-019-13533-3. PubMed
Optogenetic Activation of beta-Endorphin Terminals in the Medial Preoptic Nucleus Regulates Female Sexual Receptivity. Johnson C, Hong W, Micevych P. eNeuro. 2020 Jan 24;7(1). pii: ENEURO.0315-19.2019. doi: 10.1523/ENEURO.0315-19.2019. Print 2020 Jan/Feb. PubMed
Divergent Neural Pathways Emanating from the Lateral Parabrachial Nucleus Mediate Distinct Components of the Pain Response. Chiang MC, Nguyen EK, Canto-Bustos M, Papale AE, Oswald AM, Ross SE. Neuron. 2020 Jun 17;106(6):927-939.e5. doi: 10.1016/j.neuron.2020.03.014. Epub 2020 Apr 13. PubMed
High-fat food biases hypothalamic and mesolimbic expression of consummatory drives. Mazzone CM, Liang-Guallpa J, Li C, Wolcott NS, Boone MH, Southern M, Kobzar NP, Salgado IA, Reddy DM, Sun F, Zhang Y, Li Y, Cui G, Krashes MJ. Nat Neurosci. 2020 Oct;23(10):1253-1266. doi: 10.1038/s41593-020-0684-9. Epub 2020 Aug 3. PubMed
Improving hindlimb locomotor function by Non-invasive AAV-mediated manipulations of propriospinal neurons in mice with complete spinal cord injury. Brommer B, He M, Zhang Z, Yang Z, Page JC, Su J, Zhang Y, Zhu J, Gouy E, Tang J, Williams P, Dai W, Wang Q, Solinsky R, Chen B, He Z. Nat Commun. 2021 Feb 3;12(1):781. doi: 10.1038/s41467-021-20980-4. PubMed
Long-Range GABAergic Inhibition Modulates Spatiotemporal Dynamics of the Output Neurons in the Olfactory Bulb. Villar PS, Hu R, Araneda RC. J Neurosci. 2021 Apr 21;41(16):3610-3621. doi: 10.1523/JNEUROSCI.1498-20.2021. Epub 2021 Mar 9. PubMed
An excitatory lateral hypothalamic circuit orchestrating pain behaviors in mice. Siemian JN, Arenivar MA, Sarsfield S, Borja CB, Erbaugh LJ, Eagle AL, Robison AJ, Leinninger G, Aponte Y. Elife. 2021 May 27;10. pii: 66446. doi: 10.7554/eLife.66446. PubMed
Lateral hypothalamic LEPR neurons drive appetitive but not consummatory behaviors. Siemian JN, Arenivar MA, Sarsfield S, Borja CB, Russell CN, Aponte Y. Cell Rep. 2021 Aug 24;36(8):109615. doi: 10.1016/j.celrep.2021.109615. PubMed
Posterodorsal Medial Amygdala Regulation of Female Social Behavior: GABA versus Glutamate Projections. Johnson CS, Hong W, Micevych PE. J Neurosci. 2021 Oct 20;41(42):8790-8800. doi: 10.1523/JNEUROSCI.1103-21.2021. Epub 2021 Sep 1. PubMed
Modulation of tactile feedback for the execution of dexterous movement. Conner JM, Bohannon A, Igarashi M, Taniguchi J, Baltar N, Azim E. Science. 2021 Oct 15;374(6565):316-323. doi: 10.1126/science.abh1123. Epub 2021 Oct 14. PubMed
Inositol Polyphosphate-5-Phosphatase K (Inpp5k) Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma. Kauer SD, Fink KL, Li EHF, Evans BP, Golan N, Cafferty WBJ. J Neurosci. 2022 Mar 16;42(11):2190-2204. doi: 10.1523/JNEUROSCI.0897-21.2022. Epub 2022 Feb 8. PubMed
An Optrode Array for Spatiotemporally Precise Large-Scale Optogenetic Stimulation of Deep Cortical Layers in Non-human Primates. Angelucci A, Clark A, Ingold A, Reiche C, Iii DC, Balsor J, Federer F, McAlinden N, Cheng Y, Rolston J, Rieth L, Dawson M, Mathieson K, Blair S. Res Sq. 2023 Feb doi: 10.21203/ Preprint. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.