A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery.
Hung ME, Leonard JN
J Extracell Vesicles. 2016 May 13;5:31027. doi: 10.3402/jev.v5.31027. eCollection 2016.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168208 | pMEH31 Lamp2b HA | Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag |
| 168209 | pMEH60 Lamp2b MS2 HA | Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag |
| 168210 | pMEH275 NLS dTomato | Mammalian expression of nuclear-localized dTomato |
| 168211 | pMEH280 NLS dTomato 3x high affinity MS2 loops | Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops |
| 168212 | pMEH286 mTFP1 | Mammalian expression of mTFP1 |
| 168213 | pMEH294 NLS dTomato 1x high affinity MS2 loop | Mammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loop |
| 168214 | pMEH295 NLS dTomato 1x wild type MS2 loop | Mammalian expression of nuclear-localized dTomato with 1 wild type MS2 loop |
| 168215 | pMEH303 NLS dTomato 6x high affinity MS2 loops | Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops |
| 168216 | pMEH305 HA NLS SpCas9 3x high affinity MS2 loops | Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag |
| 168217 | pMEH343 CD63 MS2 HA | Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag |
| 168218 | pMEH361 Hspa8 MS2 HA | Mammalian expression of heat shock 70 kDa protein 8 fused to the MS2 dimer and C-terminal HA tag |
| 168219 | pMEH365 Hspa8 HA | Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag |
| 168220 | pMEH371 CD63 HA | Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag |
| 168221 | pMEH373 dTomato 3x high affinity MS2 loops | Mammalian expression of dTomato with 3 high affinity MS2 loops |
| 168222 | pMEH380 VSV-G HA | Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag |
| 168223 | pMEH381 VSV-G MS2 HA | Mammalian expression of vesicular stomatitis virus glycoprotein fused to the MS2 dimer and C-terminal HA tag |