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pDONR223_BCL6_WT

Plasmid

#81869

Purpose

Gateway Donor vector containing BCL6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BCL6 (BCL6 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R160C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL713

Plasmid

#140383

Purpose

Expresses CtcS protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CtcS-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neoBeta2mcherry

Plasmid

#45097

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 mcherry (Chrnb2 Mouse)

Use

Tags

has c-myc tag

Expression

Mammalian

Mutation

β2 was cloned into pCI-neo at the restriction sit…

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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SW5

Plasmid

#40108

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Bistable switch using dynamic degradation

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCB1a

Plasmid

#34939

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
FnR9-10

Plasmid

#182567

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-CImf

Plasmid

#40126

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lac inducible lambda repressor

Use

Synthetic Biology

Tags

mf-lon ssrA tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAG424-10X-tRNA

Plasmid

#70125

Purpose

Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Use

Tags

Expression

Yeast

Mutation

Promoter

None – SUP4 pol III promoter provided by insert

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

Plasmid

#157623

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5 (KIR2DL5A Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo:alpha4-mcherry

Plasmid

#45073

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

alpha4-mcherry (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPatL1_I

Plasmid

#146544

Purpose

Mammalian Expression of HsPatL1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1 (PATL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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RNASEL gRNA (BRDN0001149212)

Plasmid

#77051

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEL

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RNASEL (RNASEL Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RNASEL gRNA (BRDN0001149358)

Plasmid

#77052

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEL

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RNASEL (RNASEL Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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Thy1.1-G5

Plasmid

#234839

Purpose

pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

Depositor

Gabriel Victora

Article

Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

Insert

Thy1.1 (Thy1 Mouse)

Use

Retroviral

Tags

EGFP-P2A-5xGly

Expression

Mammalian

Mutation

Promoter

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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HP17deltaP8

Plasmid

#235447

Purpose

Helper plasmid for regulated phage transfer

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-PA-BAX(mito)

Plasmid

#228942

Purpose

Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus and C-terminus are deleted. BAX(3-171)…

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mTSP33

Plasmid

#236541

Purpose

For expression of Tspan33 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN33 (Tspan33 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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