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pFA6a URA3

Plasmid

#61924

Purpose

Expression of S. cerevisiae URA3 cloned into pFA6a

Depositor

Jon Houseley, David Tollervey

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

URA3

Use

Yeast genomic targeting

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET21b FAST-PETase

Plasmid

#225760

Purpose

Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

FAST-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-CUL3

Plasmid

#19893

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 3 (CUL3 Human)

Tags

3xmyc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

Plasmid

#188768

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 His6-mEGFP-D4H

Plasmid

#226373

Purpose

Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4H (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast empty (w263-1)

Plasmid

#17486

Purpose

3rd gen lentiviral empty control vector, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

Plasmid

#50946

Purpose

An empty gRNA expression vector

Depositor

Kosuke Yusa

Article

Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2014

Availability

Academic Institutions and Nonprofits only

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TK4-EGFP

Plasmid

#239047

Purpose

PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

Depositor

Marius Wernig

Article

Uenaka et al bioRxiv 2025.04.07.647695

Inserts

EGFP
puroR

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#20972

Purpose

H2B fused to mCherry for chromatin visualization

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-EGFP (BPK1098)

Plasmid

#133962

Purpose

CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

Depositor

Benjamin Kleinstiver

Insert

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP(1-10)

Plasmid

#70219

Purpose

Expresses GFP(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has Service

Cloning Grade DNA

Insert

GFP(1-10)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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mKeima-Red-Mito-7

Plasmid

#56018

Purpose

Localization: Mitochondria, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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Lamp1-mNeonGreen

Plasmid

#98882

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RLUC POLIRES FLUC

Plasmid

#45642

Purpose

Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

Depositor

Nahum Sonenberg

Article

Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

Insert

RLUC - IRES - FLUC

Use

Luciferase

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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