SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway.
Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria SM, Harper JW, Gygi SP, Sabatini DM
Science. 2017 Nov 10;358(6364):813-818. doi: 10.1126/science.aao3265. PubMed Article
Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria SM, Harper JW, Gygi SP, Sabatini DM
Science. 2017 Nov 10;358(6364):813-818. doi: 10.1126/science.aao3265. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100508 | pRK5-FLAG-SAMTOR | Expresses FLAG tagged SAMTOR in mammalian cells |
| 100509 | pRK5-FLAG-S6K1 | Expresses FLAG tagged S6K1 in mammalian cells |
| 100510 | pLJM1-FLAG-metap2 | Expresses FLAG tagged metap2 in mammalian cells to make lentivirus |
| 100511 | pLJM1-FLAG-Kaptin | Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus |
| 100512 | pRK5-HA-metap2 | Expresses HA tagged metap2 in mammalian cells |
| 100513 | pRK5-HA-SAMTOR | Expresses HA tagged SAMTOR in mammalian cells |
| 100514 | pRK5-HA-SAMTOR (G172A) | Expresses HA tagged SAMTOR G172A mutant in mammalian cells |
| 100515 | pRK5-HA-SAMTOR (D190A) | Expresses HA tagged SAMTOR D190A mutant in mammalian cells |
| 100516 | pRK5-Myc-metap2 | Expresse Myc tagged metap2 in mammalian cells |
| 100517 | pRK5-Myc-SAMTOR | Expresses Myc tagged SAMTOR in mammalian cells |
| 100518 | pLC242-GFP-metap2 | Expresses GFP tagged metap2 in mammalian cells to make lentivirus |
| 100519 | pLC242-GFP-Sestrin2 | Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus |
| 100520 | pLC242-GFP-SAMTOR | Expresses GFP tagged SAMTOR in mammalian cells to make lentivirus |
| 100521 | pCW57.1-MAT2A | Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector) |
| 102420 | pLJC5-Flag-SAMTOR | 3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter |