pLJM1-FLAG-metap2
(Plasmid #100510)

• Purpose: Expresses FLAG tagged metap2 in mammalian cells to make lentivirus
• Depositing Lab: David Sabatini
• Publication: Gu et al Science. 2017 Nov 10;358(6364):813-818. doi: 10.1126/science.aao3265.

Backbone

• Vector backbone: pLJM1
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: METAP2
• Alt name: MNPEP
• Alt name: P67EIF2
• Alt name: MAP2
• Species: H. sapiens (human)
• GenBank ID: NP_001317175.1
• Entrez Gene: METAP2 (a.k.a. MAP2, MNPEP, p67eIF2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pLJM1-F
• 3′ sequencing primer: pLJM1-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry