161,252 results

1. pLenti-EF1a-hMLH1dn-eGFP

   Plasmid

   #191104

   Purpose

   Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IRES-GFP FoxG1

   Plasmid

   #14935

   Depositor

   Joan Massague

   Article

   Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

   Insert

   FoxG1 (FOXG1 Human)
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY026

   Plasmid

   #84741

   Purpose

   Expresses huAsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PylRS-AS

   Plasmid

   #137908

   Purpose

   For incorporation of fluorophenylalanine derivatives

   Depositor

   Wenshe Liu

   Article

   Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Containing N346A, C348S mutations

   Promoter

   pBAD

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS3Clux

   Plasmid

   #55172

   Purpose

   lux-reporter vector, integration at sacA, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR hH1-TERRA

   Plasmid

   #111381

   Purpose

   TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   hH1-TERRA
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   hH1

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBAtC

   Plasmid

   #78097

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Basta resistance

   Depositor

   Jin-Soo Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NLS-HA

   Expression

   Plant

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

   Plasmid

   #110572

   Purpose

   Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   rhaSR-PrhaBAD-stRBS-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-PIG-empty

   Plasmid

   #105594

   Purpose

   empty control vector with GFP marker and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   no insert, empty control vector
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ADORA2B-DuET

    Plasmid

    #213175

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA2B (ADORA2B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO-FLAG-CHK1

    Plasmid

    #86875

    Purpose

    To express CHK1 with a FLAG tag at N-terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    CHEK1 (CHEK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p2T-cmv-SpCas9-TadCBEd-BlastR

    Plasmid

    #193848

    Purpose

    Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEYFP-C3-HA-Ubiquitin

    Plasmid

    #28236

    Depositor

    Michael Mancini

    Article

    Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    EYFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CP1041-ABE8e

    Plasmid

    #138493

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OptoEphB2-mCherry

    Plasmid

    #92042

    Purpose

    Expresses OptoEphB2 labeled with mCherry

    Depositor

    Ji Yu

    Insert

    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXIP-SHMY-PAUF

    Plasmid

    #182645

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    Pancreatic adenocarcinoma upregulated factor (PAUF)
    

    Use

    Retroviral

    Tags

    His6-tag, N-terminal ER targeting sequence of hem…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIII/MS2-1

    Plasmid

    #220629

    Purpose

    Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge