|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13421||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3600
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Growth instructionsNeeds strain with lacIq
Copy numberLow Copy
Gene/Insert nameRNAP alpha-Gal4
Insert Size (bp)900
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGA GCC TGA TAA AAA CGG TTA GCG (Common Sequencing Primers)
Terms and Licenses
Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.
This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC-Kan-alphaGal4 was a gift from Keith Joung (Addgene plasmid # 13421 ; http://n2t.net/addgene:13421 ; RRID:Addgene_13421)