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(Plasmid #13480)


Item Catalog # Description Quantity Price (USD)
Plasmid 13480 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Needs strain with lacIq
  • Copy number
    Low Copy


  • Gene/Insert name
  • Tag / Fusion Protein
    • Gal11P (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BsgI (not destroyed)
  • 5′ sequencing primer GGG GGA TTT CTG TTC ATG GGG G
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bacterial cell-based
two-hybrid (B2H) expression vector. This plasmid is a corrected version of pGP-FB (Plasmid 13419).

Note that the BsgI site (not pictured in the map below) cuts at base 420.

pGP-FF requires a bacterial strain, such as XL1 Blue, that expresses the lacIq
allele of lac repressor. This ensures repression of the strong lacUV5 promoter,
which directs expression of the zinc finger-Gal11P hybrid protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-FF was a gift from Keith Joung (Addgene plasmid # 13480 ; ; RRID:Addgene_13480)